ELISA MAX™ Deluxe Set Mouse CXCL1

Pricing & Availability
Regulatory Status
RUO
Other Names
Mouse KC, Mouse keratinocytes-derived chemokine.
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ELISA-MAX_Deluxe_Human_CXCL1
  • ELISA-MAX_Deluxe_Human_CXCL1
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447504 5 plates £209
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Description

Mouse CXCL1, a small peptide chemokine that belongs to the CXC subfamily, is produced and secreted by a variety of cells including macrophages, T cells, and epithelial. CXCL1 mediates its function by binding to glycosaminoglycans (GAGs) and the receptor CXCR2. Although CXCL1 has an important role in inflammatory responses by recruiting and activating neutrophils, aberrant expression of CXCL1 was also reported to associate with growth and progression of certain tumors such as breast, lung, and gastric cancers.

BioLegend’s ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The kit contains a polyclonal capture antibody and biotinylated polyclonal antibody specific for mouse CXCL1. BioLegend’s ELISA MAX™ Deluxe Set contains the components necessary for the accurate quantification of natural and recombinant mouse CXCL1. The set is designed for cost-effective and accurate quantification of mouse CXCL1 in serum, plasma or other biological fluids. BioLegend’s ELISA MAX™ Deluxe Sets are sensitive, accurate, and robust.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Mouse CXCL1 ELISA MAX™ Capture Antibody (200X)
  • Mouse CXCL1 ELISA MAX™ Detection Antibody (200X)
  • Mouse CXCL1 Standard Lyophilized
  • Blocking Buffer A (2X)
  • Avidin-HRP (1000X)
  • Substrate Solution D
  • Coating Buffer B (5X)
  • Assay Diluent B (5X)

Product Details

Verified Reactivity
Mouse
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
1.04 pg/mL
Standard Range
12.5 – 800 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
  • Deionized (DI) water
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Cell Type
Epithelial cells, Leukocytes, Macrophages, Neutrophils, Th17
Biology Area
Cancer Biomarkers, Immuno-Oncology, Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
2919 View all products for this Gene ID
UniProt
View information about CXCL1 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 1    Revision Date: 05/05/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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