- 4B12 (See other available formats)
- Regulatory Status
- Other Names
- C-C chemokine receptor type 7 (CCR7), MIP-3 beta receptor, EBV-induced G protein coupled receptor 1, EBI-1, CD197
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD197 is also known as C-C chemokine receptor 7 (CCR7) or EBI-1. CCR7 is a G-coupled rhodopsin-like member of the GPCR superfamily with a predicted molecular weight of 43 kD that is expressed on hematopoietic stem cells, most naive T cells, some memory T cells, B subset, and mature dendritic cells. CCR7 is a receptor for the chemokines CCL19 (MIP3 beta) and SLC (6CKine, Exodus-2, TCA-4, CCL21) that plays a role in thymocytes development, T cell adhesion at intestinal sites, the homeostatic recirculation of memory T cells, and chemotaxis.Product Details
- Antibody Type
- Host Species
- Mouse CCR7 transfected RBL-2H3 cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
The 4B12 antibody does not inhibit binding of ligand to receptor. Additional reported applications (for the relevant formats) include: immunoprecipitation. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302) is recommended prior to immunofluorescent staining.
Staining with clone 4B12 is recommended at 37°C (see supplemental data of PE staining at differing temperatures).
(PubMed link indicates BioLegend citation)
- Ohl L, et al. 2004. Immunity 21:279.
- Ritter U, et al. 2004. J. Leukocyte Biol. 76:472.
- Lan YY, et al. 2005. Am. J. Transplant. 5:2649. (FC)
- Lee JH, et al. 2007. J. Immunol. 178:301. (FC) PubMed
- Kurooka M and Kaneda Y. 2007. Cancer Res. 67:227. (FC) PubMed
- Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC)
- Sakai N, et al. 2006. P. Natl. Acad. Sci. USA 103:14098. (FC)
- Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC)
- Hwang IY, et al. 2007. J. Immunol. 179:439. (FC) PubMed
- Kang SG, et al. 2007. J. Immunol. 179:3724.
- Mao A et al. 2005. J. Immunol. 175:5146. PubMed
- Allende ML, et al. 2008. FASEB J. 22:307. PubMed
- Kang SG, et al. 2007. J. Immunol. 179:3724. PubMed
- Chen H, et al. 2005. J. Immunol. 175:591. PubMed
- Florido M, et al. 2009. Immunobiology. 214:643. PubMed
- Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
- del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
- Product Citations
AB_2715777 (BioLegend Cat. No. 120125)
- Rhodopsin-like GPCR superfamily, G-protein coupled receptor 1 family, integral membrane protein, predicted molecular weight 43 kD
Hematopoietic stem cells, T subsets, mature dendritic cells
- Homozygous mutation. Receptor for MIP-3 beta and SLC chemokines. Probable mediator of EBV effects on B lymphocytes. Plays a role in T cell adhesion at intestinal sites; may also play a role in the homeostatic recirculation of memory T cells and chemotaxis.
- MIP3 beta, SLC (6CKine, Exodus-2, TCA-4)
- Cell Type
- Hematopoietic stem and progenitors, T cells, Dendritic cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
1. Schweickart VL, et al. 1994. Genomics 23:643.
2. Yoshida R, et al. 1998. J. Biol. Chem. 273:7118.
3. Campbell JJ, et al. 1998. J. Cell Biol. 141:1053.
4. Willimann K, et al. 1998. Eur. J. Immunol. 28:2025.
- Gene ID
- 12775 View all products for this Gene ID
- View information about CD197 on UniProt.org
- Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
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