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Brilliant Violet 421™ anti-rat CD90/mouse CD90.1 (Thy-1.1) Antibody

Pricing & Availability
Clone
OX-7 (See other available formats)
Regulatory Status
RUO
Other Names
Thy-1, Thy-1.1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
OX-7_BV421_1_062311
FVB mouse splenocytes were stained with CD90.1 (clone OX-7) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • OX-7_BV421_1_062311
    FVB mouse splenocytes were stained with CD90.1 (clone OX-7) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • OX-7_BV421_2_062311
    C57BL/6 mouse splenocytes were stained with CD90.1 (clone OX-7) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
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202529 125 µL £133
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Description

CD90, also known as Thy-1, is a 28-30 kD GPI-linked membrane glycoprotein. It is a member of the immunoglobulin superfamily and has been shown to interact with CD45 in signal transduction during lymphocyte proliferation and differentiation. CD90 is expressed on hematopoietic stem cells, neurons, thymocytes, peripheral T cells, fibroblasts, stromal cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat, Mouse
Reported Reactivity
Rabbit, Guinea Pig
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat thymocyte Thy-1 antigen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The OX-7 antibody reacts with rat CD90 and mouse CD90.1 (Thy-1.1) (which is expressed by mouse strains of AKR/J, PL, and FVB/N), but not mouse CD90.2.

Additional reported applications (for the relevant formats) include: immunohistochemical7 and immunofluorescent8 staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, immunoprecipitation1, Western blotting1, in vitro activation of leukocytes2, induction of endothelial cell permeability3, induction of apoptosis in glomerular mesangial cells, and induction of glomerulonephritis in vivo4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jeng CJ, et al. 1998. J. Cell Biol. 140:685. (IP, WB)
  2. Nakashima I, et al. 1991. J. Immunol. 147:1153.
  3. Ishizu A, et al. 1995. Int. Immunol. 7:1939.
  4. Eitner F. 1997. Kidney. Int. 51:69.
  5. Kawachi H, et al. 1992. Clin. Exp. Immunol. 88:399. (WB)
  6. Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
  7. Daniel C, et al. 2012. Lab Invest. 92:812. (IHC-P)
  8. Li B, et al. 2006. Kidney Int. 69:323. (ICC)
  9. Uchimura H, et al. 2005. J Am Soc Nephrol. 16(4):997-1004. (IHC-F)
  10. Inagi R, et al. 2008. J Am Soc Nephrol. 19(5):915-22. (IHC-P)
Product Citations
  1. Wang D, et al. 2018. Immunity. 48:659. PubMed
  2. Wu J, et al. 2020. Cell Reports. 31(1):107484. PubMed
  3. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
  4. Dodagatta-Marri E, et al. 2021. Cell Rep. 36:109309. PubMed
  5. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  6. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  7. Wang D, et al. 2022. EMBO Rep. 23:e53691. PubMed
  8. Learn GD, et al. 2019. J Biomed Mater Res B Appl Biomater. 107:1864. PubMed
  9. Hong S et al. 2018. Immunity. 49(4):695-708 . PubMed
  10. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  11. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
RRID
AB_10899572 (BioLegend Cat. No. 202529)

Antigen Details

Structure
Ig superfamily, GPI-anchored membrane glycoprotein, 28-30 kD
Distribution

Hematopoietic stem cells, thymocytes, a subset of peripheral blood T cells, early myeloid and erythroid progenitors, immature B cells, neurons, activated endothelium, mast cells, dendritic cells, glomeruli

Function
Adhesion, signal transduction, lymphocyte co-stimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor
Interacts with CD45
Cell Type
B cells, Dendritic cells, Endothelial cells, Hematopoietic stem and progenitors, Mast cells, Neurons, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Campbell DG, et al. 1981. Biochem. J. 195:15.
2. Hosseinzadeh H, et al. 1993. J. Immunol. 150:1670.

Gene ID
21838 View all products for this Gene ID 24832 View all products for this Gene ID
UniProt
View information about CD90/CD90.1 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD90/CD90.1 Reagents Request Custom Conjugation
Description Clone Applications
PE/Cyanine7 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Purified anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC,IHC-F,ICC,IHC-P,IP,WB
FITC anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Alexa Fluor® 488 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC,IHC-F
Alexa Fluor® 647 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC,IHC-F
PerCP/Cyanine5.5 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
APC/Cyanine7 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Pacific Blue™ anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
PerCP anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
PE anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
APC anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Alexa Fluor® 700 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Biotin anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Brilliant Violet 421™ anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Brilliant Violet 650™ anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Brilliant Violet 510™ anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Brilliant Violet 605™ anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Brilliant Violet 711™ anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
PE/Dazzle™ 594 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
APC/Fire™ 750 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Alexa Fluor® 594 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 IHC-F
TotalSeq™-A0380 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 PG
TotalSeq™-B0380 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 PG
TotalSeq™-C0380 anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 PG
Brilliant Violet 785™ anti-rat CD90/mouse CD90.1 (Thy-1.1) OX-7 FC
Go To Top Version: 3    Revision Date: 12/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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