Brilliant Violet 421™ anti-mouse CD197 (CCR7) Antibody

Pricing & Availability
Clone
4B12 (See other available formats)
Regulatory Status
RUO
Other Names
C-C chemokine receptor type 7 (CCR7), MIP-3 beta receptor, EBV-induced G protein coupled receptor 1, EBI-1, CD197
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
4B12_BV421_1_052011
C57BL/6 splenocytes were stained with CD3 FITC and CD197 (clone 4B12) Brilliant Violet 421™ (top) or rat IgG2a Brilliant Violet 421™ (bottom).
  • 4B12_BV421_1_052011
    C57BL/6 splenocytes were stained with CD3 FITC and CD197 (clone 4B12) Brilliant Violet 421™ (top) or rat IgG2a Brilliant Violet 421™ (bottom).
  • 4B12_BV421_2_052011
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
120119 125 µL £133.00
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120120 500 µL £253.00
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Description

CD197 is also known as C-C chemokine receptor 7 (CCR7) or EBI-1. CCR7 is a G-coupled rhodopsin-like member of the GPCR superfamily with a predicted molecular weight of 43 kD that is expressed on hematopoietic stem cells, most naive T cells, some memory T cells, B subset, and mature dendritic cells. CCR7 is a receptor for the chemokines CCL19 (MIP3 beta) and SLC (6CKine, Exodus-2, TCA-4, CCL21) that plays a role in thymocytes development, T cell adhesion at intestinal sites, the homeostatic recirculation of memory T cells, and chemotaxis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CCR7 transfected RBL-2H3 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The 4B12 antibody does not inhibit binding of ligand to receptor. Additional reported applications (for the relevant formats) include: immunoprecipitation. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302) is recommended prior to immunofluorescent staining.

Staining with clone 4B12 is recommended at 37°C (see supplemental data of PE staining at differing temperatures).

Application References

(PubMed link indicates BioLegend citation)
  1. Ohl L, et al. 2004. Immunity 21:279.
  2. Ritter U, et al. 2004. J. Leukocyte Biol. 76:472.
  3. Lan YY, et al. 2005. Am. J. Transplant. 5:2649. (FC)
  4. Lee JH, et al. 2007. J. Immunol. 178:301. (FC) PubMed
  5. Kurooka M and Kaneda Y. 2007. Cancer Res. 67:227. (FC) PubMed
  6. Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC)
  7. Sakai N, et al. 2006. P. Natl. Acad. Sci. USA 103:14098. (FC)
  8. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC)
  9. Hwang IY, et al. 2007. J. Immunol. 179:439. (FC) PubMed
  10. Kang SG, et al. 2007. J. Immunol. 179:3724.
  11. Mao A et al. 2005. J. Immunol. 175:5146. PubMed
  12. Allende ML, et al. 2008. FASEB J. 22:307. PubMed
  13. Kang SG, et al. 2007. J. Immunol. 179:3724. PubMed
  14. Chen H, et al. 2005. J. Immunol. 175:591. PubMed
  15. Florido M, et al. 2009. Immunobiology. 214:643. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Product Citations
  1. Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed
  2. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  3. Mariani SA, et al. 2019. Immunity. 50:1439. PubMed
  4. Chen H, et al. 2005. J Immunol. 175:591. PubMed
  5. Hwang I, Kehrl C 2004. J Immunol. 179:439. PubMed
  6. Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
RRID
AB_10897811 (BioLegend Cat. No. 120119)
AB_2561446 (BioLegend Cat. No. 120120)

Antigen Details

Structure
Rhodopsin-like GPCR superfamily, G-protein coupled receptor 1 family, integral membrane protein, predicted molecular weight 43 kD
Distribution

Hematopoietic stem cells, T subsets, mature dendritic cells

Function
Homozygous mutation. Receptor for MIP-3 beta and SLC chemokines. Probable mediator of EBV effects on B lymphocytes. Plays a role in T cell adhesion at intestinal sites; may also play a role in the homeostatic recirculation of memory T cells and chemotaxis.
Ligand/Receptor
MIP3 beta, SLC (6CKine, Exodus-2, TCA-4)
Cell Type
Hematopoietic stem and progenitors, T cells, Dendritic cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Schweickart VL, et al. 1994. Genomics 23:643.
2. Yoshida R, et al. 1998. J. Biol. Chem. 273:7118.
3. Campbell JJ, et al. 1998. J. Cell Biol. 141:1053.
4. Willimann K, et al. 1998. Eur. J. Immunol. 28:2025.

Gene ID
12775 View all products for this Gene ID
UniProt
View information about CD197 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 4    Revision Date: 12/05/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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