- 3.9 (See other available formats)
- Regulatory Status
- III NL707
- Other Names
- Integrin αX subunit, CR4, p150, ITGAX
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin αX and CR4. CD11c non-covalently associates with integrin β2 (CD18) and is expressed on monocytes/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.Product Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon, Chimpanzee, Squirrel Monkey
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Clone 3.9 preferentially binds the activated form of CD11c, is specific for the I domain of CD11c, and is able to partially block the binding of CD11c and ICAM-4. 3.9 binding is divalent cation dependent12. While analyzing blood, it is best to use heparin as the anti-coagulant and not EDTA. Since the ability of clone 3.9 to bind to its target is divalent cation dependent, the usage of EDTA as an anti-coagulant may be detrimental to staining due to its chelating properties.
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4, and functional assays5,6. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301616). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 301632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
(PubMed link indicates BioLegend citation)
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York.
- McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
- Vainer B, et al. 2000. Am. J. Surg. Pathol. 24:1115. (IHC)
- Ottonello L, et al. 1999. Blood 93:3505.
- Metelitsa LS, et al. 2002. Blood 99:4166.
- Sadhu C, et al. 2007. J. Leukoc. Biol. doi:10.1189/jlb.1106680. PubMed
- Ihanus E, et al. 2007. Blood 109:802-810.
- Gurer C, et al. 2008. Blood 112:1231. PubMed
- Asai A, et al. 2009. J. Lipid Res. 50:95. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Sadhu C, et al. 2008. J. Immunoass. Immunoch. 29:42. (FC)
- Product Citations
AB_10898313 (BioLegend Cat. No. 301627)
AB_11203895 (BioLegend Cat. No. 301628)
- Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 145-150 kD
Myeloid, dendritic cells, NK cells, B cells and T cell subsets
- Adhesion, CTL killing
- CD54, fibrinogen, iC3b, ICAM-1, ICAM-4
- Cell Type
- B cells, Dendritic cells, Neutrophils, NK cells, T cells, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Petty H. 1996. Immunol. Today 17:209.
2. Springer T. 1994. Cell 76:301.
3. Ihanus E, et al. 2007. Blood 109:802-810.
- Gene ID
- 3687 View all products for this Gene ID
- View information about CD11c on UniProt.org
- What is the F/P ratio range of our BV421™ format antibody reagents?
It is lot-specific. On average it ranges between 2-4.
|FITC anti-human CD11c||3.9||FC|
|PE anti-human CD11c||3.9||FC|
|Purified anti-human CD11c||3.9||FC, CyTOF®, IHC|
|PE/Cyanine7 anti-human CD11c||3.9||FC|
|PE/Cyanine5 anti-human CD11c||3.9||FC|
|Biotin anti-human CD11c||3.9||FC|
|APC anti-human CD11c||3.9||FC|
|Alexa Fluor® 488 anti-human CD11c||3.9||FC|
|Alexa Fluor® 647 anti-human CD11c||3.9||FC|
|Pacific Blue™ anti-human CD11c||3.9||FC|
|PerCP/Cyanine5.5 anti-human CD11c||3.9||FC|
|Brilliant Violet 421™ anti-human CD11c||3.9||FC|
|Brilliant Violet 711™ anti-human CD11c||3.9||FC|
|Ultra-LEAF™ Purified anti-human CD11c||3.9||FC, CyTOF®, Block, IHC|
|Brilliant Violet 510™ anti-human CD11c||3.9||FC, ICC|
|Brilliant Violet 605™ anti-human CD11c||3.9||FC|
|Brilliant Violet 650™ anti-human CD11c||3.9||FC|
|Purified anti-human CD11c (Maxpar® Ready)||3.9||FC, CyTOF®|
|PE/Dazzle™ 594 anti-human CD11c||3.9||FC|
|Brilliant Violet 785™ anti-human CD11c||3.9||FC|
|Alexa Fluor® 700 anti-human CD11c||3.9||FC|
|APC/Fire™ 750 anti-human CD11c||3.9||FC|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-human CD11c
PE anti-human CD11c
Purified anti-human CD11c
PE/Cyanine7 anti-human CD11c
PE/Cyanine5 anti-human CD11c
Biotin anti-human CD11c
APC anti-human CD11c
Alexa Fluor® 488 anti-human CD11c
Alexa Fluor® 647 anti-human CD11c
Pacific Blue™ anti-human CD11c
PerCP/Cyanine5.5 anti-human CD11c
Brilliant Violet 421™ anti-human CD11c
Brilliant Violet 711™ anti-human CD11c
Ultra-LEAF™ Purified anti-human CD11c
Brilliant Violet 510™ anti-human CD11c
Brilliant Violet 605™ anti-human CD11c
Brilliant Violet 650™ anti-human CD11c
Purified anti-human CD11c (Maxpar® Ready)
PE/Dazzle™ 594 anti-human CD11c
Brilliant Violet 785™ anti-human CD11c
Alexa Fluor® 700 anti-human CD11c
APC/Fire™ 750 anti-human CD11c