Brilliant Violet 421™ anti-GATA3 Antibody

Pricing & Availability
16E10A23 (See other available formats)
Regulatory Status
Other Names
GATA-binding factor 3, trans-acting T-cell-specific transcription factor GATA-3
Mouse IgG2b, κ
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Product Citations
Human T leukemia cell line Jurkat (filled histogram) or Burkitt's lymphoma cell line Ramos (open histogram) were treated with FOXP3 Fix/Perm Buffer Set, and then stained with anti-human GATA3 (clone 16E10A23) Brilliant Violet 421™.
  • 16E10A23_BV421_GATA3_Antibody_FC_022014.jpg
    Human T leukemia cell line Jurkat (filled histogram) or Burkitt's lymphoma cell line Ramos (open histogram) were treated with FOXP3 Fix/Perm Buffer Set, and then stained with anti-human GATA3 (clone 16E10A23) Brilliant Violet 421™.
Compare all formats See Brilliant Violet 421™ spectral data
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653813 25 tests £125
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653814 100 tests £291
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GATA3 functions as a major regulator of T helper 2 (Th2) cell differentiation in immune cells. GATA3 expression is upregulated through IL-4 receptor signaling or the Notch-mediated pathway, which results in production of IL-4, IL-5, and IL-13 cytokines, responsible for preferential differentiation of Th2-cells. GATA3 has been shown to regulate early developmental processes of T cells, such as T cell commitment, positive selection, and post-commitment CD4+ T cell survival. In the mammary gland, GATA3 plays an important role in differentiation and commitment of luminal epithelial cells. In a mouse model, exogenous expression of GATA3 in undifferentiated breast carcinoma cells induces differentiation and suppresses spreading of the tumor cells, suggesting that GATA3 is involved in preventing malignancy of breast cancer. GATA3 has been reported to be essential in the development of a variety of normal tissues. Defect in GATA3 results in hypoparathyroidism, sensorineural deafness, and renal dysplasia (HDR syndrome).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Host Species
Partial human GATA3 recombinant protein (1-258 aa).
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Product Citations
  1. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  2. Cho SX, et al. 2020. Nat Commun. 4.481944444. PubMed
  3. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
  4. Giampazolias E, et al. 2021. Cell. . PubMed
  5. Feng J,et al. 2017. Nat Commun. . 10.1038/s41467-017-01056-8. PubMed
  6. Green CD et al. 2018. Developmental cell. 46(5):651-667 . PubMed
  7. Gu BH, et al. 2019. J Immunol. 203:349. PubMed
  8. Di Blasi D, et al. 2019. Cell Mol Gastroenterol Hepatol. 0.510416667. PubMed
  9. Kim M, et al. 2018. Immunity. 49:151. PubMed
AB_2563220 (BioLegend Cat. No. 653813)
AB_2563221 (BioLegend Cat. No. 653814)

Antigen Details

48 kD protein containing two GATA-type zinc finger domains responsible for DNA binding and two trans-activating domains at N-terminus.


GATA3, a transcriptional activator, binds to a consensus DNA sequence 5'-(A/T)GATA(A/G)-3' in the promoter region of genes, regulates T cell development, TH2 cell differentiation, and luminal cell proliferation.
Interacts with Smad3, FOG1, FOG2, and PU.1.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Yagi R, et al. 2011. Int. Immunol. 23:415.
2. Chou J, et al. 2010. J. Cell Physiol. 222:42.
3. Ho IC, et al. 2009. Nat. Rev. Immunol. 9:125.
4. Rothenberg EV, et al. 2008. Semin. Immunol. 20:236.
5. Kouros-Mehr H, et al. 2008. Curr. Opin. Cell Biol. 20:164.
6. Van Esch H, et al. 2000. Nature 406:419.

Gene ID
2625 View all products for this Gene ID 14462 View all products for this Gene ID
View information about GATA3 on

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 02/24/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • PE anti-GATA3

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  • PerCP/Cyanine5.5 anti-GATA3

  • Brilliant Violet 421™ anti-GATA3


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