- Poly5188 (See other available formats)
- Regulatory Status
- Other Names
- Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
- Goat Polyclonal Ig
- Ave. Rating
- Submit a Review
- Product Citations
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Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Recombinant rat IFN-γ
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by antigen affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
ELISA Detection - Quality tested
WB - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.1 - 0.5 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of rat IFN-γ recombinant protein ranging from 2000 to 31 pg/mL are recommended for each ELISA plate. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA Detection: The biotinylated poly5188 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified DB-1 antibody (Cat. No. 507801 or 507808) as the capture antibody and recombinant rat IFN-γ (Cat. No. 580009) as the standard.
Note: For testing rat IFN-γ in serum, plasma or cell culture supernatant, BioLegend's ELISA Legend Max™ Kits (Cat. No. 439007 & 439008) are specially developed and recommended.
- Product Citations
AB_2561418 (BioLegend Cat. No. 518803)
- Cytokine, dimer, 40-80 kD (Mammalian)
- CD8+ and CD4+ T cells, NK cells
- Upregulated by IL-2, bFGF, EGF, downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
- T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
- IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
- Cell Type
- T cells, NK cells
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Antigen References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.
- Gene ID
- 25712 View all products for this Gene ID
- View information about IFN-gamma on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
|Biotin anti-rat IFN-γ||Poly5188||ELISA Detection, WB|
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