Biotin anti-human IL-5 Antibody

Pricing & Availability
Clone
JES1-5A10 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T cell replacing factor-1 (TRF-1)
Isotype
Rat IgG2a, κ
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Product Citations
publications
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501002 500 µg £157.00
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Description

IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. The JES1-39D10 antibody can neutralize the bioactivity of natural or recombinant IL-5.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity*: Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS-expressed, recombinant human IL-5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection, ELISPOT Detection

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-5 protein ranging from 250 to 1 pg/ml are recommended for each ELISA plate when paired with TRFK5 (Cat. # 504302). To obtain a linear standard curve, serial dilutions of human IL-5 protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate when paired with JES1-39D10 (Cat. # 500902).
For use as an ELISPOT detection antibody, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA1-3 or ELISPOT4-6 Detection: The purified JES1-5A10 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated JES1-39D10 (Cat. No. 501002) as the detection antibody. Each lot of this antibody is quality control tested by ELISA assay.
Neutralization1: The LEAF™ Purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-5 bioactivity.
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining8,9 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.
Note: For testing human IL-5 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430401 & 430404) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  2. Butterfield J, et al. 1992. Blood 79:688.
  3. Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133.
  4. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19.6.
  5. Mahanty S, et al. 1992. J. Immunol. 148:3567.
  6. Limaye A, et al. 1991. J. Clin. Inv. 88:1418.
  7. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
  8. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  9. Andersson J, et al. 1994. Immunology 83:16.
Product Citations
  1. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
RRID
AB_315144 (BioLegend Cat. No. 501002)

Antigen Details

Structure
Cytokine; dimer; 45 kD (Mammalian)
Bioactivity
Growth/differentiation of eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
Cell Sources
Mast cells, T cells, bone marrow stromal cells
Cell Targets
Eosinophils
Receptors
Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Takatsu K, et al. 1988. Immunol. Rev. 102:107.
3. Takatsu K. 1992. Curr. Opin. Immunol. 4:299.
4. Takatsu K. 1991. Microbiol. Immun. 35:593.

Regulation
Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID
3567 View all products for this Gene ID
UniProt
View information about IL-5 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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