- NOK-1 (See other available formats)
- Regulatory Status
- VII 70322
- Other Names
- Fas Ligand (FasL), CD95L, TNFSF6, Fas-L
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD178 is a 38-42 kD type II glycoprotein also known as Fas ligand and CD95L. CD178 belongs to the TNF superfamily and is expressed on activated T lymphocytes, NK cells, monocytes, and granulocytes. CD178 is also expressed on parenchymal cells of the retina and cornea, retinal pigment epithelial cells, and testis. The extracellular region of FasL can be cleaved by matrix metalloproteinases (MMPs) to give rise to a 26 kD soluble protein. CD178 binds to CD95, a member of the TNFR superfamily, to induce apoptosis. CD95/CD95L interactions play an important role in the maintenance of peripheral tolerance and survival.Product Details
- Verified Reactivity
- Reported Reactivity
- Antibody Type
- Host Species
- L5178Y mouse T lymphoma cells expressing recombinant human FasL
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, immunofluorescence microscopy3, immunocytochemistry2, blocking of Fas induced apoptosis1, and Western blotting11. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 306407) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 306404) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). In addition, applying matrix metalloproteinases (MMPS) inhibitor in the cell culture system will increase the FasL staining intensity. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 306415 and 306416).
(PubMed link indicates BioLegend citation)
- Kayagaki N, et al. 1995. J. Exp. Med. 182:1777.
- Herr I, et al. 2000. Cell Death Differ. 7:129. (WB)
- Bossi G, et al. 1999. Nature Medicine 5:90.
- Andreola G, et al. 2002. J. Exp. Med. 195:1303.
- Strauss L, et al. 2009. J. Immunol. 182:1469. PubMed
- Li JH, et al. 2009. Am J. Pathol. 175:1124. PubMed
- Zhao Q, et al. 2011. Fitoterapia. 82:735. PubMed
- Kruger K, et al. 2011. J. Appl. Physiol. 110:1226. PubMed
- Qin G, et al. 2012. J. Infect. Dis. PubMed
- Khalid M, et al. 2012. J. Virol. 86:4906. PubMed
- Qin G, et al. 2012. J. Infect. Dis. 205:1646. PubMed
- Shrestha B, et al. 2012. J. Virol. 86:8937. PubMed
- Mooren FC, et al. 2012. J. Appl. Physiol.113:1082. PubMed
- Robinet P, et al. 2014. J Immunol. 192:5332. PubMed
- Wang Y, et al. 2014. J Endocrinol. 222:151. PubMed
AB_314602 (BioLegend Cat. No. 306404)
- TNF superfamily, type II glycoprotein, 38-42 kD, 26 kD soluble form
Activated T cells, NK cells, testis, eye, neutrophils, Clara type II cells
- Apoptosis, immune privilege
- Cell Type
- Neutrophils, NK cells, T cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
1. Suda T, et al. 1997. J. Exp. Med. 12:204.
2. Kayagaki N, et al. 1995. J. Exp. Med. 182:1777.
3. Tanaka M, et al. 1995. EMBO J. 14:1129.
- Gene ID
- 356 View all products for this Gene ID
- View information about CD178 on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
|Biotin anti-human CD178 (Fas-L)||NOK-1||FC|
|PE anti-human CD178 (Fas-L)||NOK-1||FC|
|Purified anti-human CD178 (Fas-L)||NOK-1||FC, ICC, IP, WB|
|Brilliant Violet 421™ anti-human CD178 (Fas-L)||NOK-1||FC|
|TotalSeq™-A0177 anti-human CD178 (Fas-L)||NOK-1||PG|
|Ultra-LEAF™ Purified anti-human CD178 (Fas-L)||NOK-1||FC, ICC, IP, WB|
|PE/Cyanine7 anti-human CD178 (Fas-L)||NOK-1||FC|
|TotalSeq™-C0177 anti-human CD178 (Fas-L) Antibody||NOK-1||PG|
|APC anti-human CD178 (Fas-L)||NOK-1||FC|
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Biotin anti-human CD178 (Fas-L)
PE anti-human CD178 (Fas-L)
Purified anti-human CD178 (Fas-L)
Brilliant Violet 421™ anti-human CD178 (Fas-L)
TotalSeq™-A0177 anti-human CD178 (Fas-L)
Ultra-LEAF™ Purified anti-human CD178 (Fas-L)
PE/Cyanine7 anti-human CD178 (Fas-L)
TotalSeq™-C0177 anti-human CD178 (Fas-L) Antibody
APC anti-human CD178 (Fas-L)