Biotin anti-APP, 573-596 Antibody (Previously Covance catalog# SIG-39181)

Pricing & Availability
Clone
1G6 (See other available formats)
Regulatory Status
RUO
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously
Covance Catalog# SIG-39181
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
IG6_Biotin_APP573-596_Antibody_1_021819.png
Western blot of Biotin anti-APP, 573-596 antibody (clone 1G6). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain lysate; Lane 3: 40 µg of mouse brain lysate; Lane 4: 40 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP conjugated streptavidin. Enhanced chemiluminescence was used as the detection system.
  • IG6_Biotin_APP573-596_Antibody_1_021819.png
    Western blot of Biotin anti-APP, 573-596 antibody (clone 1G6). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain lysate; Lane 3: 40 µg of mouse brain lysate; Lane 4: 40 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP conjugated streptavidin. Enhanced chemiluminescence was used as the detection system.
  • IG6_Biotin_APP573-596_Antibody_2_021819.png
    Western blot of Biotin anti-APP, 573-596 antibody (clone 1G6). Lane 1: Molecular weight marker; Lane 2: 0.01 µg of recombinant human APP770 protein. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP conjugated streptavidin. Enhanced chemiluminescence was used as the detection system.
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806004 200 µL £431
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Description

Amyloid beta (Aβ or Abeta) denotes peptides of 36–43 amino acids in length that are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. The peptides result from the amyloid precursor protein (APP), which is cut by certain enzymes to yield Aβ. Aβ molecules can aggregate to form oligomers (known as "seeds") which are believed to be able to induce other Aβ molecules to also take the misfolded oligomeric form, leading to a chain reaction akin to a prion infection. The seeds or the resulting amyloid plaques are toxic to nerve cells. The other protein implicated in Alzheimer's disease, tau protein, also forms such prion-like misfolded oligomers, and there is some evidence that misfolded Aβ can induce tau to misfold.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The epitope lies within amino acids 573-596 of amyloid precursor protein. Cross-reactivity to fragments may be expected based on the location of the epitope.

Additional reported applications (for the relevant formats) include: immunoprecipitation2.

Application References

(PubMed link indicates BioLegend citation)
  1. Halford RW and Russell DW. Reduction of cholesterol synthesis in the mouse brain does not affect amyloid formation in Alzheimer's disease, but does extend lifespan. PNAS; 106: 3502 - 3506, Mar 2009. (WB)
  2. Welzel AT, et al. 2014. Biochemistry 24:3908. (IP)
Product Citations
  1. Grant MKO, et al. 2019. PLoS One. 14:e0212815. PubMed
RRID
AB_2564694 (BioLegend Cat. No. 806004)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Gene ID
351 View all products for this Gene ID
UniProt
View information about APP 573-596 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 4    Revision Date: 02/18/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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