APC anti-mouse TER-119/Erythroid Cells Antibody

Pricing & Availability
Clone
TER-119 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-76
Isotype
Rat IgG2b, κ
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Product Citations
publications
TER-119_APC_122107
C57BL/6 bone marrow cells stained with TER-119 APC and anti-mouse CD45 FITC
  • TER-119_APC_122107
    C57BL/6 bone marrow cells stained with TER-119 APC and anti-mouse CD45 FITC
See APC spectral data
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116211 25 µg £27.00
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116212 100 µg £86.00
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Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References

(PubMed link indicates BioLegend citation)
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Liu J, et al. 2012. PLoS One. 7:e44044. PubMed
  2. Lafkas D, et al. 2013. J Cell Biol. 203:47. PubMed
  3. Song J, et al. 2014. Exp Cell Res. 327:123. PubMed
  4. Khoriaty R, et al. 2014. Mol Cell Biol. 34:3721. PubMed
  5. Yin Y, et al. 2016. J Biol Chem. 291: 6923 - 6935. PubMed
  6. Yeo S, et al. 2016. Cancer Res . 76: 3397 - 3410. PubMed
  7. Rowe R, et al. 2016. J Exp Med. 213: 1497 - 1512. PubMed
  8. Cambridge E, et al. 2017. Exp Hematol. 45:64-68.e5. PubMed
  9. DeSouza-Vieira T, et al. 2020. Cell Rep. 33:108317. PubMed
  10. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  11. Liu P, et al. 2020. Genome Res. 30:1655. PubMed
  12. Markworth JF, et al. 2020. JCI Insight. 5:00. PubMed
  13. Wang H, et al. 2020. JCI Insight. 5:00. PubMed
  14. Werner MT, et al. 2020. J Biol Chem. 295:1898. PubMed
  15. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
  16. Nayak RR, et al. 2021. Cell Host Microbe. 29(3):362-377.e11. PubMed
  17. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  18. Koury D, et al. 2017. Int J Parasitol. 10.1016/j.ijpara.2017.05.009. PubMed
  19. Pal B, et al. 2017. Nat Commun.. 10.1038/s41467-017-01560-x. PubMed
  20. Lam J, et al. 2018. Nat Commun. 9:2418. PubMed
  21. Wang L, et al. 2018. Bone Res. 6:22. PubMed
  22. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  23. Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed
  24. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  25. Parada-Kusz M, et al. 2018. Dis Model Mech. 11:. PubMed
  26. Wang H, et al. 2019. J Clin Invest. 129:598. PubMed
  27. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  28. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  29. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  30. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  31. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  32. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  33. Carmody C et al. 2019. Endocrinology. 160(5):1205-1222 . PubMed
  34. Fu NY, et al. 2018. Dev Cell. 47:629. PubMed
  35. Riffelmacher T et al. 2017. Immunity. 47(3):466-480 . PubMed
  36. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  37. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  38. Mourao L, et al. 2019. Sci Rep. 0.991666667. PubMed
  39. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  40. Lutzmann M, et al. 2019. Cell Rep. 28:2851. PubMed
  41. Siddique SM, et al. 2019. Sci Rep. 9:13977. PubMed
  42. Li G, et al. 2020. J Clin Invest. 130:1233. PubMed
  43. Ray S, et al. 2020. J Biol Chem. 295:7113. PubMed
  44. Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed
  45. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  46. Shin JJ, et al. 2020. Cell Rep. 32:108093. PubMed
  47. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  48. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  49. Yeo SK, et al. 2020. eLife. 9:e58810.. PubMed
  50. Engelbrecht E, et al. 2020. eLife. 9:e52690.. PubMed
  51. Bilodeau M, et al. 2009. PLoS One. 4:e7500. PubMed
  52. Guihard S, et al. 2010. Blood. 115:3686. PubMed
RRID
AB_313712 (BioLegend Cat. No. 116211)
AB_313713 (BioLegend Cat. No. 116212)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 12/04/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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