Anti-Synaptobrevin VAMP Antibody anti-Synaptobrevin (VAMP)

Pricing & Availability

Clone: SP10 (See other available formats)
Regulatory Status: RUO
Other Names: Vesicle-associated membrane protein 1, synaptobrevin-1
Previously: Covance Catalog# MMS-616R
Isotype: Mouse IgG2b, κ
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Product Citations: publications

Western blot of anti-Synaptobrevin (VAMP) antibody (clone SP10). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with a 1:2000 dilution of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgM. Enhanced chemiluminescence was used as the detection system.

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837001

500 µL

£299

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Description

Synaptobrevins, also known as VAMPs, along with syntaxins and the synaptosomal-associated protein SNAP25, form the protein complex that is involved with the docking and fusion of synaptic vesicles with the presynaptic membrane. Synaptobrevins have a molecular weight of 18 kD, and are also involved in the formation of the SNARE complexes. The synaptobrevins are degraded by the protein tetanospasmin, from the tetanus-causing bacterium Clostridium tetani, as well as botulinum toxin from Clostridium botulinum.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human, Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

This antibody was raised against a crude synaptic preparation of post mortem human brain.

Preparation

Ascites

Concentration

The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.

Storage & Handling

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.

Application

WB - Quality tested
IHC-P, ELISA, ICC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution of this reagent is 1:1000 - 1:2000. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

SP10 reacts with native, denatured and recombinant synaptic vesicle proteins synaptobrevin/VAMP-1 (aa 1-118) and VAMP-2 expressed in a bacterial system (aa 33-96). The antibody is particularly valuable for immunocytochemical studies of the brain due to its stability in formalin sections.

Application References (PubMed link indicates BioLegend citation)

Sawada K, et al. 2002. Mol Psychiatry 7:484-92. (WB, IHC-P, ELISA)
Honer WG, et al. 2002. Cerebral Cortex 12:349-56. (WB, ELISA, ICC)
Honer WG, et al. 1993. Brain Res. 609:9-20. (ICC)
Poon VY, et al. 2014. Front. Cell Neurosci. 8:66. (ICC) PubMed

Product Citations

Poon V, et al. 2014. Front Cell Neurosci. 8:66. PubMed

RRID

AB_2565369 (BioLegend Cat. No. 837001)

Antigen Details

Structure: Synaptobrevin 1 (VAMP1) is a 118 amino acid protein with an apparent molecular mass of 13kD. Synaptobrevin 2 (VAMP2) is a 116 amino acid protein with an apparent molecular mass of 12.5 kD.
Biology Area: Cell Biology, Neuroscience, Synaptic Biology
Molecular Family: Presynaptic proteins
Gene ID: 6843 View all products for this Gene ID
UniProt: View information about Synaptobrevin on UniProt.org

Documentation

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Related Protocols

Western Blotting Protocol

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Other Formats

View All Synaptobrevin (VAMP) Reagents Request Custom Conjugation

Description	Clone	Applications
Anti-Synaptobrevin (VAMP)	SP10	WB,IHC-P,ELISA,ICC
Purified anti-Synaptobrevin (VAMP)	SP10	WB,IHC-P,ELISA,ICC

Certificate of Analysis

Go To Top Version: 4 Revision Date: 10/29/2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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