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Anti-Fibrillarin Antibody (Previously Covance catalog# MMS-581S)

Pricing & Availability
Clone
38F3 (See other available formats)
Regulatory Status
RUO
Other Names
RRNA 2'-O-methyltransferase fibrillarin, 34 kD nucleolar scleroderma antigen, RNA, U3 small nucleolar interacting protein 1
Previously
Covance Catalog# MMS-581S
Isotype
Mouse IgG1
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Product Citations
publications
1_38F3_PURE_Fibrillarin_Antibody_1_WB_112817
Western blot analysis of cell lysates from A549, HEL (human), UMR106 (rat) and Raw264.7 (mouse) cells using Fibrillarin mouse primary antibody (clone 38F3, 1:1000 dilution) and HRP Goat anti-mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control (1:8000 dilution).
  • 1_38F3_PURE_Fibrillarin_Antibody_1_WB_112817
    Western blot analysis of cell lysates from A549, HEL (human), UMR106 (rat) and Raw264.7 (mouse) cells using Fibrillarin mouse primary antibody (clone 38F3, 1:1000 dilution) and HRP Goat anti-mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control (1:8000 dilution).
  • 1_38F3_PURE_Fibrillarin_Antibody_2_WB_112817
    Total lysates (15 µg protein) from 293T and 293T/Fibrillarin CRISPR/Cas9 knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1ug/mL) purified anti-Fibrillarin antibody, clone 38F3 (upper) or 1:3000 diluted anti-GAPDH (poly6314, Cat. No. 631401) antibody (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP (Cat. No. 405306) for anti-Fibrillarin antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP (Cat. No. 406401) for GAPDH. Lane M: MW ladder.
  • 2_38F3_Fibrillarin_Antibody_IHC_021615
    Human HEK293 cell stained with mouse monoclonal antibody to fibrillarin 38F3 (green). DNA is stained with Hoechst.
  • 4_38F3_PURE_Fibrillarin_Antibody_4_WB_010620
    Western blot of purified anti-Fibrillarin antibody (clone 38F3). Lane 1: Molecular weight marker; Lane 2: 20 µg of Drosophila S2 (embryonic) cell lysate. The blot was incubated with a 1:500 dilution of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 5_38F3_PURE_Fibrillarin_Antibody_5_ICC_010620
    ICC staining of anti-Fibrillarin antibody (clone 38F3) on Drosophila S2 cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with a 1:500 dilution of the primary antibody overnight at 4°C, followed by incubation wit 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI. The right image shows costaining with Flash Phalloidin™ Green 488 (Cat No. 424201). The images were captured with a 60X objective. Scale bar: 20 µm
Cat # Size Price Quantity Check Availability Save
905001 200 µL £215
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Description

Nop1p was originally identified as a nucleolar protein of bakers yeast, S. cerevisiae (accession P15646). The Nop1p protein is 327 amino acids in size (34.5kD), is essential for yeast viability, and is localized in the nucleoli. It is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein apparently found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. This means that suitably cross-reactive antibodies to Nop1p/fibrillarin, like MCA-38F3, can be used to reveal nucleoli and study fibrillarin/Nop1p in all eukaryotes and archea tested to date.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Drosophila
Reported Reactivity
Other species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
38F3 was raised against yeast nuclear preparations.
Formulation
Contains 0.01M sodium azide.
Preparation
Tissue Culture Supernatant
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
ICC, KO/KD-WB  - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 0.5 µg per mL (dilution 1:500 - 1:1000). For immunocytochemistry, a dilution range of 1:500 (mammalian), and 1:1000 - 1:5000 (yeast) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Reactivity to Drosophila was only verified with the purified format.

This antibody is useful for immunoblotting (WB), immunofluorescence (ICC) and immunocytochemistry.

*Predicted MW = 34 kD

Additional Product Notes

For Western blotting, the suggested use of this reagent is 1:500-1:2000 dilution in Drosophila and 1:500-1:1000 dilution in other species. For immunocytochemistry, a dilution range of 1:500 (mammalian), 1:100 - 1:500 (Drosophila) and 1:1000 - 1:5000 (yeast) is recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Newton K, et al. 2003. Mol. Cell Biol. 23:8519.
  2. Ochs RL, et al. 1985. Biol. Cell. 54:123.
  3. Lessard F, et al. 2012. Nucelic Acids Res. 40:5357. (IF) PubMed
  4. Scruggs BS. et al. 2012. Mol Cell Biol. 32:4092. (WB) PubMed
  5. Lessard F, et al. 2010. Moll Cell. 38:539. (IF, WB)
  6. Denissov S, et al. 2011. EMBO Rep. 12:231. (IF) PubMed
Product Citations
  1. Lessard F, et al. 2012. Nucleic Acids Res. 40:5357-5367. PubMed
  2. Mars JC, et al. 2020. NAR Cancer. 2:zcaa032. PubMed
  3. Herdman C, et al. 2017. PLoS Genet.. 10.1371/journal.pgen.1006899. PubMed
  4. Scruggs B, et al. 2012. Mol Cell Biol. 32:4092-4103. PubMed
  5. Denissov S, et al. 2011. EMBO Rep. 12:231-237. PubMed
  6. Tomita T, et al. 2021. Nat Commun. 12:3655. PubMed
RRID
AB_2565045 (BioLegend Cat. No. 905001)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
2091 View all products for this Gene ID
UniProt
View information about Fibrillarin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 9    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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