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Alexa Fluor® 700 anti-human CD328 (Siglec-7) Antibody

Pricing & Availability
Clone
6-434 (See other available formats)
Regulatory Status
RUO
Workshop
VIII 80652
Other Names
Siglec7 (Sialic-binding Ig like lectin 7), p75, AIRM1
Isotype
Mouse IgG1, κ
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Product Citations
publications
6-434_A700_CD328_Antibody_062819.png
Human peripheral blood lymphocytes were stained with CD56 Brilliant Violet 421™ and CD328 (Siglec-7) (clone 6-434) Alexa Fluor® 700 (left) or Mouse IgG1, κ Alexa Fluor® 700 isotype control (right).
  • 6-434_A700_CD328_Antibody_062819.png
    Human peripheral blood lymphocytes were stained with CD56 Brilliant Violet 421™ and CD328 (Siglec-7) (clone 6-434) Alexa Fluor® 700 (left) or Mouse IgG1, κ Alexa Fluor® 700 isotype control (right).
Compare all formats See Alexa Fluor® 700 spectral data
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339209 25 tests £109
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339210 100 tests £262
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Description

Siglec-7, also known as p75/AIRM1, is a 75 kD type I transmembrane protein and a member of the family of sialic acid-binding immunoglobulin-like lectins (Siglecs). It is primarily found on NK cells and monocytes. The cytoplasmic domain of Siglec-7 contains immunoreceptor tyrosine-based inhibitory motif (ITIM). CD328 mediates sialic acid-dependent cell-cell binding and functions as an inhibitory receptor of NK cells. CD328 preferentially binds to sialylated glycans with α2,8 disialyl and α2,6 sialyl residues.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
RRID
AB_2814245 (BioLegend Cat. No. 339209)
AB_2814246 (BioLegend Cat. No. 339210)

Antigen Details

Structure
Type I transmembrane protein, Siglec family member, 75 kD
Distribution

NK cells, monocytes

Function
Inhibitory receptor of NK cells
Ligand/Receptor
Sialylated glycans (with α2,8 disialyl and α2,6 sialyl residues)
Cell Type
Monocytes, NK cells
Biology Area
Immunology
Molecular Family
CD Molecules, Siglec Molecules
Antigen References
  1. Avril T, et al. 2006. Infection and Immunity 74:4133
  2. Avril T, et al. 2004. J. Immunol. 173:6841
  3. Yamaji T, et al. 2005. Glycobiology 15:667
Gene ID
27036 View all products for this Gene ID
UniProt
View information about CD328 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/28/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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