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Alexa Fluor® 488 anti-human Cadherin 11 Antibody

Pricing & Availability
Clone
16G5 (See other available formats)
Regulatory Status
RUO
Other Names
OSF-4, Osteoblast cadherin, OB-cadherin, CDH11, CDHOB, CAD11
Isotype
Mouse IgG1, κ
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Product Citations
publications
16G5_A488_Cadherin11_Antibody_1_111319
Human paraffin-embedded kidney tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with BioLegend’s Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. The tissue was washed with PBS/ 0.05% Tween 20 twice for five minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-Cadherin 11 (clone 16G5) Alexa Fluor®488 (green) and anti-Nestin (clone 10C2) Alexa Fluor®647 (red) at 4°C overnight. This image was captured with a 10X objective.
  • 16G5_A488_Cadherin11_Antibody_1_111319
    Human paraffin-embedded kidney tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with BioLegend’s Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. The tissue was washed with PBS/ 0.05% Tween 20 twice for five minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-Cadherin 11 (clone 16G5) Alexa Fluor®488 (green) and anti-Nestin (clone 10C2) Alexa Fluor®647 (red) at 4°C overnight. This image was captured with a 10X objective.
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368712 100 µg £254
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Description

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin 11 is a type I transmembrane glycoprotein. It is a type II (atypical) cadherin, based on its lack of a HAV cell adhesion recognition sequence that is specific to type I cadherins. The expression of this particular cadherin in osteoblastic cell lines along with its upregulation during differentiation suggests a specific function in bone development and maintenance. This cadherin is also expressed in some cancer cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein containing the extracellular cadherin domain 1 of Cadherin 11 fused to maltose binding protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation.

RRID
AB_2686939 (BioLegend Cat. No. 368712)

Antigen Details

Structure
Type I single pass transmembrane glycoprotein.
Distribution

Osteoblasts, neuroblasts, and some cancer cells.

Function
Cell adhesion.
Interaction
Cadherin 2 (CDH2).
Ligand/Receptor
Calcium.
Cell Type
Osteoclasts
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules
Antigen References

1. Wheelock MJ, Johnson KR. 2003. Annu. Rev. Cell Dev. Biol. 19:207-35.
2. Christofori G, et al. 2003. EMBO. J. 22:2318.
3. Okazaki M, et al. 1994. J. Biol. Chem. 269:12092.

Gene ID
1009 View all products for this Gene ID
UniProt
View information about Cadherin 11 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/08/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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