Ultra-LEAF Purified anti-mouse CD19 Antibody, CD19, 6D5

Pricing & Availability

Clone: 6D5 (See other available formats)
Regulatory Status: RUO
Other Names: B4
Isotype: Rat IgG2a, κ
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Product Citations: publications

6D5_LEAF_080207 — C57BL/6 mouse splenocytes were stained with Ultra-LEAF™ purified CD19 (clone 6D5) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG FITC.

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115569

100 µg

88€

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115570

1 mg

542€

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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details

Technical data sheet

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse CD19-expressing K562 human erythroleukemia cells

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
CyTOF® - Verified
IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence⁷.

Application References (PubMed link indicates BioLegend citation)

Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
Olive V, et al. 2013. Elife. 2:822. PubMed
Miyai T, et al. 2014. PNAS. 111:11780. PubMed

RRID

AB_2832390 (BioLegend Cat. No. 115569)
AB_2832391 (BioLegend Cat. No. 115570)

Antigen Details

Structure: Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution: Pro-B cells to mature B cells (during development), follicular dendritic cells
Function: Modulates B cell activation and differentiation
Ligand/Receptor: CD21, CD81, Leu-13
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.
Gene ID: 12478 View all products for this Gene ID
UniProt: View information about CD19 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Does BioLegend test each Ultra-LEAF™ antibody by functional assay?: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?: No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD19 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse CD19	6D5	FC
Biotin anti-mouse CD19	6D5	FC
FITC anti-mouse CD19	6D5	FC
PE anti-mouse CD19	6D5	FC
PE/Cyanine5 anti-mouse CD19	6D5	FC
Purified anti-mouse CD19	6D5	FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19	6D5	FC
Alexa Fluor® 488 anti-mouse CD19	6D5	FC
Alexa Fluor® 647 anti-mouse CD19	6D5	FC,IHC-F
Pacific Blue™ anti-mouse CD19	6D5	FC
Alexa Fluor® 700 anti-mouse CD19	6D5	FC
APC/Cyanine7 anti-mouse CD19	6D5	FC
PerCP anti-mouse CD19	6D5	FC
PerCP/Cyanine5.5 anti-mouse CD19	6D5	FC
Alexa Fluor® 594 anti-mouse CD19	6D5	IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19	6D5	FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19	6D5	FC
Brilliant Violet 605™ anti-mouse CD19	6D5	FC
Brilliant Violet 650™ anti-mouse CD19	6D5	FC
Brilliant Violet 785™ anti-mouse CD19	6D5	FC
Brilliant Violet 510™ anti-mouse CD19	6D5	FC
Purified anti-mouse CD19 (Maxpar® Ready)	6D5	FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD19	6D5	FC
Brilliant Violet 711™ anti-mouse CD19	6D5	FC
APC/Fire™ 750 anti-mouse CD19	6D5	FC
TotalSeq™-A0093 anti-mouse CD19	6D5	PG
Brilliant Violet 750™ anti-mouse CD19	6D5	FC
TotalSeq™-B0093 anti-mouse CD19	6D5	PG
Spark Blue™ 550 anti-mouse CD19	6D5	FC
Spark NIR™ 685 anti-mouse CD19	6D5	FC
TotalSeq™-C0093 anti-mouse CD19	6D5	PG
Ultra-LEAF™ Purified anti-mouse CD19	6D5	FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody	6D5	FC
Spark YG™ 581 anti-mouse CD19	6D5	FC
APC/Fire™ 810 anti-mouse CD19	6D5	FC
Spark YG™ 570 anti-mouse CD19	6D5	IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody	6D5	FC
Spark Blue™ 515 anti-mouse CD19	6D5	FC
Spark UV™ 387 anti-mouse CD19	6D5	FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™)	6D5	FC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 03.15.2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.