Ultra-LEAF™ Purified anti-human BMP9 (GDF2) Antibody

Pricing & Availability
Clone
A16035H (See other available formats)
Other Names
Bone morphogenetic protein 9 (BMP9), growth differentiation factor 2 (GDF2)
Isotype
Mouse IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
Human_BMP9_RECOM_022318
Recombinant human BMP9 (Cat. No. 553102) induces alkaline phosphatase in ATDC5 mouse chondrogenic cell line in a dose dependent manner (black circles). Anti-human BMP9 monoclonal antibody (clone A16035H, purple circles) neutralizes the production of alkaline phosphatase in ATDC5 cells induced by BMP9 (2 ng/mL). The ND50= 0.05 - 0.25 µg/mL.
  • Human_BMP9_RECOM_022318
    Recombinant human BMP9 (Cat. No. 553102) induces alkaline phosphatase in ATDC5 mouse chondrogenic cell line in a dose dependent manner (black circles). Anti-human BMP9 monoclonal antibody (clone A16035H, purple circles) neutralizes the production of alkaline phosphatase in ATDC5 cells induced by BMP9 (2 ng/mL). The ND50= 0.05 - 0.25 µg/mL.
Cat # Size Price Quantity Avail. Save
611703 100 µg 196€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
611704 1 mg 520€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

BMP9 was initially isolated from fetal mouse liver cDNA libraries. The human and mouse proteins share 84% identity. They are part of the bone morphogenetic protein (BMP) family of proteins, which are members of the TGF-β superfamily and play a key role in skeletal development, bone formation, and stem cell differentiation. BMP9 is one of the most osteogenic factors of the family. It is sinthetized by hepatocytes and intrahepatic biliary epithelial cells and circulates in plasma as an unprocessed inactive form that can be further activated by furin, and as a mature and fully active form as a complex associated to its prodomain. BMPs signal through heterotetrameric complex receptors, which include four type I receptors (ALK1, ALK2, ALK3 and ALK6) and three type II receptors (BMPRII, ActRIIA and ActRIIB). BMP9 and -10 bind to ALK1 (mainly expressed in endothelial cells). BMP9 inhibits the proliferation of endothelial cells induced by basic FGF and the VEGF-stimulated angiogenesis by its binding to ALK1. BMP9 also binds to ALK2 in primary myeloma cells, and this binding can be inhibited by membranal or soluble endoglin (accessory receptor for the TGFβ family). BMP9 activity is inhibited by crossveinless2 (CV2), a member of the chordin family and extracellular regulator. BMP9 induces CV2 expression in endothelial cells and in a negative feedback loop inhibits its own activity by the binding of CV2 to BMP9, and consequently inhibits its binding to ALK1. BMP9 and -10 possess tumor suppressing activity in breast and prostate cancer; BMP9 as well induces apoptosis in primary myeloma cells. In contrast, autocrine BMP9 signaling induces proliferation of ovarian and liver cancer cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant BMP9
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by neutralizing activity. The ND50 is 0.05 - 0.25 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2728466 (BioLegend Cat. No. 611703)
AB_2728467 (BioLegend Cat. No. 611704)

Antigen Details

Structure
Homodimer
Distribution

Hepatocytes, non parenchimal cells of the liver, endothelial, stellate cells, osteosarcoma cells.

Function
Induces osteogenesis, chondrogenesis, differentiation of cholinergic neurons, and regulates glucose metabolism.
Interaction
Endothelial cells,microvascular endothelial cells, prostate cancer cells, ovarian cancer cells, osteosarcoma cells, primary myeloma cells.
Ligand/Receptor
Actvin receptor-like kinase- ALK-1 and ALK-5 in endothelial cells; ALK1 and BMPR-II in endothelial cells; ALK-1 and ALK-2 in mesenchymal stem cells; ALK2 and BMPR-II in myoblasts; ALK1 and endoglin in glioblastoma cells. Endoglin (CD105).
Cell Type
Endothelial cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Gene ID
2658 View all products for this Gene ID
UniProt
View information about BMP-9 on UniProt.org

Related FAQs

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 0    Revision Date: 02.23.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Forgot your password? Reset password?
Create an Account