Recombinant Human VEGF (ELISA Std.)

Pricing & Availability
Regulatory Status
RUO
Other Names
VEGFA, VEGF-A, VPF, MVCD1, vascular endothelial growth factor
Ave. Rating
Submit a Review
Product Citations
publications
RECOM_HU_VEGF_ELISA_STD_121118.PNG
  • RECOM_HU_VEGF_ELISA_STD_121118.PNG
Cat # Size Price Quantity Check Availability Save
560309 4 pack 76€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

VEGF (also known as VEGF-A) is a signaling protein with strong vascular permeability activity that stimulates the formation of new blood vessels during the processes of vasculogenesis and angiogenesis. It is a member of the vascular endothelial growth factor family, which in mammals is comprised of VEGF-A, VEGF-B, VEGF-C, VEGF-D, and PlGF (placental growth factor). There are multiple isoforms of VEGF that result from alternative splicing of mRNA. VEGF binds and activates two tyrosine kinase receptors, VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1), through which VEGF exerts its mitogenic effects. VEGF is highly expressed in solid tumors of breast, lung, renal, colorectal, and hepatic origin. Additionally, it significantly contributes to ascites tumor formation. Beyond cancer, VEGF has also been associated with multiple pathogenic conditions including rheumatoid arthritis, age-related macular degeneration, Crow-Fukase syndrome, sclerosis and Alzheimer’s disease.

Product Details
Technical data sheet

Product Details

Source
Human VEGF-165, amino acids Ala27-Arg191 (Accession# AAM03108) was expressed in 293E cells.
Molecular Mass
The 165 amino acid recombinant protein has a predicted molecular mass of approximately 19 kD. The DTT-reduced protein migrates at approximately 20-28 kD and non-reduced protein migrates at 50 kD by SDS-PAGE. The N-terminal amino acid is Alanine.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles.
Application

ELISA - Quality tested

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 23.4 to 1,500 pg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This human VEGF protein is useful as a standard for a human VEGF sandwich ELISA, using unlabeled A15136H antibody (catalog #537002) as capture and biotinylated Poly5225 antibody (catalog #522503) as detection.

Antigen Details

Structure
Homodimer
Distribution

Widely expressed

Function
VEGFA is a key player in vasculogenesis, the formation of blood vessels from progenitor cells, as well as angiogenesis. The expression of the VEGFA gene is upregulated via hypoxia, estrogen, and NF-κB pathways. In addition, VEGFA is upregulated by PDGF-BB, P1GF, TGFβ1, IGF1, FGFs, HGF, TNFα, and IL-1. VEGFA induces proliferation and cell migration in endothelial cells, and plays important roles during wound healing. Also, VEGFA regulates haematopoietic stem cell survival.
Interaction
VEGFA interacts with vascular endothelial cells and monocytes/macrophages, which express VEGFR1. This interaction induces proliferation of endothelial cells and stimulates migration of monocytes/macrophages.
Ligand/Receptor
VEGFA binds and activates two tyrosine kinase receptors, VEGFR1 (Flt-1), VEGFR2 (KDR/Flk-1)
Biology Area
Angiogenesis, Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Conn G, et al. 1990 Proc. Natl. Acad. Sci. USA 87:1323.
  2. Gerber H, et al. 2002. Nature 417:954.
  3. Shibuya M. 2006. J. Biochem. Mol. Biol. 39:469.
  4. Shibuya M. 2008. BMB Rep. 41:278.
  5. Monaghan-Benson E, et al. 2010. The American Journal of Pathology 177:2091.
  6. Koch S and Claesson-Welsh L. 2012. Cold Spring Harb Perspect Med. 2:a006502.
Gene ID
7422 View all products for this Gene ID
UniProt
View information about VEGF on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12.11.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account