Purified anti-human Mesothelin Antibody

Pricing & Availability
MN (See other available formats)
Other Names
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
Cat # Size Price Quantity Avail. Save
530101 50 µg 100€
Check Availability

Need larger quantities of this item?
Request Bulk Quote

Human mesothelin, also known as CAK1 and MSLN, is a cell-surface protein that is expressed on normal mesothelial cells lining the pleura, pericardium, and peritoneum. It is expressed as a 70 kD pre-cursor protein with a furin cleavage site, which produces two products: 1) megakaryocyte potentiating factor (MPF) (30 kD) and 2) mesothelin (40 kD). MPF is a cytokine that is released. Mesothelin, for the most part, is membrane-bound by a glycosylphosphatidylinositol linkage and has three glycosylation sites. Soluble mesothelin is believed to result from proteolytic cleavage to release it from the membrane linkage, or from alternative splicing of the mesothelin gene.

The normal function of mesothelin is not known. Mice knockouts produced no obvious phenotypes with normal growth and reproductive parameters. However, mesothelin is overexpressed by cancer cells, particularly in mesotheliomas, ovarian adenocarcinomas, pancreatic adenocarcinomas, and squamous cell carcinomas, and lung cancers. It binds to MUC16/CA-125, which is a cell surface mucin and ovarian cancer biomarker, and has been speculated to be involved in peritoneal dissemination of ovarian cancer cells. Mesothelin has also been shown to be elevated in the serum of cancer patients compared to normal patients. As such, it has become the focus of cancer research in the areas of diagnostics, immunotherapy, and cancer vaccinology. For example, SS1P is a recombinant immunotoxin which targets mesothelin-expressing cancer cells. Its anti-mesothelin Fγ domain confers specificity, while its Pseudomonas exotoxin domain results in cell death. This potential drug has already finished Stage I clinical trials.

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
DNA immunization (mesothelin expression plasmid) with mesothelin-Fc fusion protein for final boost.
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

ELISA Capture - Quality tested
ELISA Detection, FC, IHC-P, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. A concentration of 2 µg/ml of the capture antibody was utilized to generate the example standard curve for human mesothelin. It is recommended that each lot of reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: To measure human mesothelin, this antibody can be used as a capture antibody in sandwich ELISA format, and paired with the biotinylated (clone MB) antibody (Cat. No. 520203) as the detection antibody. Recombinant Human Mesothelin (ELISA Std.) (Cat. No. 593209) can be used as the protein standard.

Note: For testing human mesothelin in serum, plasma, or supernatant, LEGEND MAX™ Human Mesothelin ELISA Kit (Cat. No. 438607 & 438608) is specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Onda M, et al. 2005. Clin. Cancer Res. 11:5840.
AB_2571908 (BioLegend Cat. No. 530101)

Antigen Details

~40 kD glycoprotein that can be membrane-bound, or relased from the membrane in a soluble form.

Mesothelial cells lining the pleura, peritoneum, and pericardium. It is also located in various cancer cells.

Not known. May promote carcinogenesis and metastasis.
Various cancer cells such as ovarian and pancreatic cancer cells.
CA125 (MUC16).
Biology Area
Cancer Biomarkers, Cell Adhesion, Cell Biology
Molecular Family
Antigen References

1. Pastan I, et al. 2014. Cancer Res. 74:2907.
2. Hellstrom I, et al. 2011. Expert Opin. Med. Diagn. 1:227.
3. Hassan R, et al. 2008. Eur. J. Cancer 44:46.
4. Hassan R, et al. 2006. Clin. Cancer Res. 2:447.
5. Onda M, et al. 2005. Clin. Cancer Res. 16:5840-6.
6. Hassan R, et al. 2004. Clin. Cancer Res. 10:3937.
7. Scholler N, et al. 1999. Proc. Natl. Acad. Sci. 96:11531.

Gene ID
10232 View all products for this Gene ID
View information about Mesothelin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02.26.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Insert Note Here
Save Close Clear
Lab Timer
Login / Register
Forgot your password? Reset password?
Create an Account