Purified anti-human CXCL10 (IP-10) Antibody

Pricing & Availability
Clone
J036G3 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon gamma-inducible protein 10 (IP10), Interferon gamma induced factor, gIP-10, IFI10, INP10
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
J036G3_PURE_CXCL10_Antibody_1_ELISA_051316
  • J036G3_PURE_CXCL10_Antibody_1_ELISA_051316
  • J036G3_PURE_CXCL10_Antibody_2_WB_051316
    Western blot analysis of 50 ng of recombinant human CXCL10 (IP-10) (lane 1) and 50 ng of recombinant mouse CXCL10 (IP-10) (lane 2) using anti-human CXCL10 (IP-10) (clone J036G3) antibody.
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524401 50 µg 68,00€
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524402 500 µg 236,00€
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Description

CXCL10 is an ELR-negative chemokine structurally and functionally related to CXCL9 and CXCL11.  CXCL10, CXCL9, and CXCL11 are produced and secreted by monocytes, macrophages, fibroblasts, and epithelial cells upon stimulation with proinflammatory cytokines, especially IFNγ. CXCL10 chemoattracts CD4, CD8, and NK and NKT cells through the binding to its receptor CXCR3, which is shared with CXCL9 and CXCL11. In addition, CXCL10 inhibits neovascularization in tumors and in wound healing in vivo. Also, CXCL10 has anti-proliferative effects on endothelial cells in vitro, and angiostatic and antitumor effects in vivo. It has been suggested that the anti-proliferative effect of CXCL10 in endothelial cells is CXCR3-independent and that it is mediated through GAG interaction. CXCL10 also possesses antimicrobial activity against E. coli and L. monocytogenes, and both the spore and bacillus forms of B. anthracis. CXCL10 expression is strongly upregulated in many human inflammatory diseases, including rheumatoid arthritis, type I diabetes, multiple sclerosis, atherosclerosis, allograft rejection, and others.     

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CXCL10
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of CXCL10 (IP-10) recombinant protein ranging from 2000 to 31.3 pg/ml are recommended for each ELISA plate. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: The purified J036G3 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5194 antibody (Cat. No. 519403) as the detecting antibody and recombinant human CXCL10 (IP-10) (Cat. No. 573509) as the standard.

Note: For testing human CXCL10 (IP-10) in serum, plasma or supernatant, BioLegend's ELISA LEGEND MAX™ Kits (Cat. No. 439907 & 439908) are specially developed and recommended.

Product Citations
  1. Gruber CN, et al. 2020. Cell. 183:982. PubMed
RRID
AB_2562398 (BioLegend Cat. No. 524401)
AB_2562399 (BioLegend Cat. No. 524402)

Antigen Details

Structure
Chemokine
Distribution
Interferon gamma-stimulated keratinocytes, monocytes, macrophages, fibroblasts, endothelial, T cells
Function
CXCL10 chemoattracts CD4, Th1, CD8, NK, and NKT cells. CXCL10 is induced by inflammatory cytokines, and NH2-terminal cleavage of CXCL10 by DPP-IV/CD26 impairs its chemoattracting capacity and CXCR3 signaling.
Interaction
Activated T cells, Th1 cells, regulatory T cells, NK cells, NKT cells, endothelial cells, fibroblasts, endothelial cells
Ligand/Receptor
CXCR3
Cell Type
Monocytes, Macrophages, Fibroblasts, Endothelial cells, T cells
Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Luster AD, et al. 1985. Nature 315:672.
2. Loos T, et al. 2008. Blood 112:2648.
3. Crawford MA, et al. 2009. Infect. Immun. 77:1664.
4. Campanella GS, et al. 2010. PLoS One 5:e12700.
5. Hoerning A. 2011. Eur. J. Immunol. 41:2291.

Gene ID
3627 View all products for this Gene ID
UniProt
View information about CXCL10 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06.01.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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