- H4A3 (See other available formats)
- Regulatory Status
- P PR-63; BP 473; P P008
- Other Names
- Lysosome-Associated Membrane Protein 1, LGP-120, LAMP-1
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40 kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation, CD107a is transferred to the cell membrane surface of activated platelets, activated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107a has been suggested to play a role in the protection of lysosomal membrane from lysosomal hydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surface expression of LAMP-1 has been shown to correlate with CD8+ T cell and NK cell cytotoxicity.Product Details
- Verified Reactivity
- Reported Reactivity
- African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
- Antibody Type
- Host Species
- Human adult adherent peripheral blood cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
ICC, WB, IHC-P - Verified
IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunocytochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 μg per ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting8, immunohistochemical staining2, immunofluorescence5,7, and immunoprecipitation5.
This antibody is specific to human LAMP-1. Positive control: Hela cells; LAMP-1 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.
(PubMed link indicates BioLegend citation)
- Misse D, et al. 1999. Blood 93:2454.
- Furuta K, et al. 2001. Am. J. Pathol. 159:449. (IHC)
- Watanabe A, et al. 2011. J. Biol. Chem. 286:10702. PubMed
- Baron Gaillard CL, et al. 2011. Mol. Cell. Biol. 22:5459. PubMed
- Hauck CR and Meyer TF. 1997. FEBS Lett. 405:86. (IF, IP)
- De Keersmaecker B, et al. 2012. J. Virol. 86:9351. PubMed
- Knodler LA, et al. 2010. P. Natl. Acad. Sci. USA. 107:17733. (IF)
- Oh J, et al. 2000. Hum. Mol. Genet. 9:375. (WB)
- Salio M, et al. 2013 PNAS. 110:4753. PubMed
- Product Citations
AB_1134260 (BioLegend Cat. No. 328601)
AB_1134259 (BioLegend Cat. No. 328602)
- LAMP-1 is a 417 amino acid protein with a molecular mass of 45 kD.
Macrophages, epithelial cells, endothelial cells, some tumor cells; located on the luminal side of lysosomes or on the surface of cell membranes
- Protect lysosomal membrane from lysosomal hydrolases, adhesion
- Cell Type
- Endothelial cells, Epithelial cells, Macrophages
- Biology Area
- Cell Biology, Immunology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Sarafian V, et al. 2006. Arch. Dermatol. Res. 298:7381.
2. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press.
3. Sawada R, et al. 1993. J. Biol. Chem. 268:12675.
4. Chen JW, et al. 1988. J. Biol. Chem. 263:8754.
5. Chen JW, et al. 1986. Biochem. Soc. Symp. 51:97112.
- Gene ID
- 3916 View all products for this Gene ID
- View information about CD107a on UniProt.org
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Biotin anti-human CD107a (LAMP-1)
Purified anti-human CD107a (LAMP-1)
FITC anti-human CD107a (LAMP-1)
PE anti-human CD107a (LAMP-1)
Alexa Fluor® 488 anti-human CD107a (LAMP-1)
Alexa Fluor® 647 anti-human CD107a (LAMP-1)
PerCP/Cyanine5.5 anti-human CD107a (LAMP-1)
APC anti-human CD107a (LAMP-1)
Pacific Blue™ anti-human CD107a (LAMP-1)
Brilliant Violet 421™ anti-human CD107a (LAMP-1)
PE/Cyanine7 anti-human CD107a (LAMP-1)
APC/Cyanine7 anti-human CD107a (LAMP-1)
Brilliant Violet 510™ anti-human CD107a (LAMP-1)
Brilliant Violet 605™ anti-human CD107a (LAMP-1)
Purified anti-human CD107a (LAMP-1) (Maxpar® Ready)
Brilliant Violet 650™ anti-human CD107a (LAMP-1)
Brilliant Violet 711™ anti-human CD107a (LAMP-1)
PerCP anti-human CD107a (LAMP-1)
Brilliant Violet 785™ anti-human CD107a (LAMP-1)
PE/Dazzle™ 594 anti-human CD107a (LAMP-1)
TotalSeq™-A0155 anti-human CD107a (LAMP-1)
TotalSeq™-C0155 anti-human CD107a (LAMP-1)
TotalSeq™-B0155 anti-human CD107a (LAMP-1)
APC/Fire™ 750 anti-human CD107a (LAMP-1) Antibody
PE/Cyanine5 anti-human CD107a (LAMP-1)