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PerCP/Cyanine5.5 anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1dot4_PerCPCy55_061909.jpg
C57BL/6 bone marrow cells stained with HK1.4 PerCP/Cyanine5.5
  • HK1dot4_PerCPCy55_061909.jpg
    C57BL/6 bone marrow cells stained with HK1.4 PerCP/Cyanine5.5
Compare all formats See PerCP/Cyanine5.5 spectral data
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128011 25 µg 113€
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128012 100 µg 268€
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Lin C et al. 2019. Immunity. 50(1):137-151 . PubMed
  2. Turecamo SE, et al. 2018. Matrix Biol. 67:01:00. PubMed
  3. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  4. Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
  5. Xiang G, et al. 2022. Cell Death Dis. 13:451. PubMed
  6. Schädlich IS, et al. 2022. iScience. 25:104470. PubMed
  7. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
  8. Ballesteros I, et al. 2014. J Vis Exp. 94: 52031. PubMed
  9. Gallizioli M, et al. 2020. Cell Rep. 33:108291. PubMed
  10. Fachi JL et al. 2019. Cell reports. 27(3):750-761 . PubMed
  11. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  12. Tang L, et al. 2021. Oncol Lett. 22:605. PubMed
  13. Israelow B, et al. 2020. bioRxiv. . PubMed
  14. Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed
  15. Zhang K, et al. 2018. PLoS Pathog. 14:e1006925. PubMed
  16. Song J, et al. 2021. J Am Heart Assoc. 10:e017329. PubMed
  17. Xu ZH, et al. 2021. Mediators Inflamm. 2021:8856326. PubMed
  18. Mylonas K, et al. 2015. Immunobiology. 220: 924-933. PubMed
  19. Leach SM, et al. 2020. Cell Rep. 33:108337. PubMed
  20. Yilmaz B, et al. 2021. Cell Host Microbe. 29(4):650-663.e9. PubMed
  21. Yang Y, et al. 2020. Cell Death Discov. 0.293055556. PubMed
  22. Huard A, et al. 2021. J Immunol. 206:1058. PubMed
  23. Demars A, et al. 2021. PLoS Pathog. 17:e1009887. PubMed
  24. Di Gioia M, et al. 2020. Nat Immunol. 21:42. PubMed
  25. Park JA, et al. 2021. J Hematol Oncol. 14:142. PubMed
  26. Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed
  27. Shemer A, et al. 2020. Immunity. 53(5):1033-1049.e7. PubMed
  28. Zhou Z, et al. 2019. Oxid Med Cell Longev. 2019:1209307. PubMed
  29. Han YH, et al. 2021. Science. 373:. PubMed
  30. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  31. McCreedy DA et al. 2018. eNeuro. 5(4) pii: ENEURO. PubMed
  32. Zhang Y, et al. 2015. PLoS Pathog. 11: e1005167. PubMed
  33. Stutchfield B, et al. 2015. Gastroenterology. 149: 1896-1909.e14. PubMed
  34. Jeljeli M, et al. 2020. Cell Rep. 33:108325. PubMed
  35. Lagares D, et al. 2017. Sci Transl Med. 9:eaal3765. PubMed
  36. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
  37. Forbester JL, et al. 2020. J Virol. 94:. PubMed
  38. Cassidy BR, et al. 2020. J Neuroinflammation. 17:259. PubMed
  39. Honarpisheh P, et al. 2020. J Neuroinflammation. 0.9625. PubMed
  40. Tanaka T, et al. 2015. J Immunol. 194:2004. PubMed
  41. Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed
  42. Horn KJ, et al. 2022. Nat Commun. 13:3321. PubMed
  43. Okubo K, et al. 2021. Cell Reports. 35(7):109142. PubMed
  44. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
  45. Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed
  46. Golden TN, et al. 2022. Front Pharmacol. 12:761496. PubMed
  47. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  48. Brigas HC, et al. 2021. Cell Reports. 36(9):109574. PubMed
  49. Paiva-Oliveira E, et al. 2017. Histochem Cell Biol. 10.1007/s00418-017-1556-6. PubMed
  50. Medler TR et al. 2018. Cancer cell. 34(4):561-578 . PubMed
  51. Cosentino K, et al. 2022. Mol Cell. 82:933. PubMed
  52. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  53. Zeng W, et al. 2021. STAR Protocols. 2(1):100361. PubMed
  54. Jamali A, et al. 2020. Cell Reports. 32(9):108099. PubMed
  55. Williams JW, et al. 2017. Circ Res. 121:662. PubMed
  56. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  57. Tang C, et al. 2021. Theranostics. 11:9791. PubMed
  58. Rashid MH, et al. 2021. Oncol Rep. 45:1171. PubMed
  59. Blriot C, et al. 2021. Immunity. :. PubMed
  60. Ermert D, et al. 2016. PLoS Pathog. 11: 1005043. PubMed
  61. Yang Y, et al. 2020. Cell Death Discov. 6:62. PubMed
  62. Wuggenig P, et al. 2020. Commun Biol. 3:130. PubMed
  63. Ali S, et al. 2021. PLoS One. 16:e0246646. PubMed
  64. Nuro-Gyina PK, et al. 2021. J Immunol. 207:868. PubMed
  65. Ioannidis L, et al. 2016. J Immunol. 196: 1227 - 1238. PubMed
  66. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  67. Pinho–Ribeiro FA, et al. 2018. Cell. 173:1083. PubMed
  68. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  69. Hadas R, et al. 2020. JCI Insight. 5:00. PubMed
  70. Teo T, et al. 2015. J Virol. 89: 7955-7969. PubMed
  71. Klein O, et al. 2019. J Allergy Clin Immunol. 144:1074. PubMed
  72. Budai Z, et al. 2021. Cells. 10:. PubMed
  73. Fontana MF, et al. 2015. Parasite Immunol. 37: 470-478. PubMed
  74. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  75. Kumari P, et al. 2021. Cell Reports. 35(3):109012. PubMed
  76. Namkoong H, et al. 2018. PLoS Pathog. 14:e1006955. PubMed
  77. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  78. Canedo T, et al. 2021. Neuropsychopharmacology. 46:2358. PubMed
  79. Nishio H, et al. 2020. Regen Ther. 14:262. PubMed
  80. Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
  81. Huo M, et al. 2017. FASEB J. 10.1096/fj.201601030R. PubMed
  82. Hendrikx S et al. 2019. Cell reports. 26(5):1227-1241 . PubMed
  83. Walsh CM, et al. 2019. Elife. 8:e48448. PubMed
  84. Foster G, et al. 2015. J Immunol. 195: 5380 - 5392. PubMed
  85. Yajjala V, et al. 2016. J Immunol. 196: 4196 - 4203. PubMed
  86. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  87. Lei Y, et al. 2021. Sci Adv. 7:. PubMed
  88. Chen X, et al. 2021. Cell Death Dis. 12:1123. PubMed
  89. Freed-Pastor WA, et al. 2021. Cancer Cell. 39:1342. PubMed
  90. Heuslein J, et al. 2016. Sci Rep. 6: 27029. PubMed
  91. Jiang Y, et al. 2015. MBio. 6: e01426-15. PubMed
  92. Valdearcos M et al. 2017. Cell metabolism. 26(1):185-197 . PubMed
  93. Orr MT, et al. 2019. NPJ Vaccines. 4:1. PubMed
  94. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
  95. Ioanna E Galani et al. 2017. Immunity. 46(5):875-890 . PubMed
  96. Boguslawski KM, et al. 2020. Sci Adv. 6:eaax7515. PubMed
  97. Joshi JC, et al. 2020. Cell Rep. 30:4096. PubMed
  98. Al-Zaeed N, et al. 2021. Cell Death Dis. 12:611. PubMed
  99. Gangoso E, et al. 2021. Cell. 184:2454. PubMed
  100. Ikezu S, et al. 2021. Mol Psychiatry. 26:1808. PubMed
  101. Awida Z, et al. 2021. Int J Mol Sci. 23:. PubMed
  102. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  103. Qi S, et al. 2020. Theranostics. 10:1814. PubMed
  104. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  105. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
  106. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
  107. Steinmetz O, et al. 2016. J Infect Dis. 10.1093/infdis/jiv763. PubMed
RRID
AB_1659242 (BioLegend Cat. No. 128011)
AB_1659241 (BioLegend Cat. No. 128012)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 12.09.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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