LEGEND MAX™ Human IGFALS ELISA Kit

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Other Names
IGFALS, ACLSD, ALS, insulin-like growth factor binding protein, acid labile subunit
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Human_IGFALS_ELISA_102318
  • Human_IGFALS_ELISA_102318
Cat # Size Price Quantity Save
445907 1 Pre-coated Plate 396€
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Description

Insulin-like growth factor (IGF) is an important hormone for childhood growth and in a variety of physiological functions in adults. Free IGF has a short half-life in serum of about 10 minutes. This half-life is extended by forming a binary complex with IGFBP1-6 to 30 - 90 minutes. IGFALS binds the IGF/IGFBP3 or IGBP5 complex to generate a ternary complex with a serum half-life of more than 12 hours. About 80% of circulating IGF is in this ternary complex.

Insulin-like growth factor binding protein, acid labile subunit (IGFALS), is produced by the liver and is present at high concentrations in postnatal serum. IGFALS is encoded by the IGFALS gene and stimulated by growth hormone. Defects in the IGFALS gene result in lower circulating levels of IGF-1 and IGF-2, causing delayed puberty, increased risk of metastatic disease, and is associated with insulin resistance. Different conditions such as fasting, catabolic disease, or cirrhosis have been shown to reduce serum IGFALS levels in humans. The IGFALS protein is composed of 578 amino acids with 20 leucine-rich repeats.

BioLegend's LEGEND MAX™ Human IGFALS ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a monoclonal mouse anti-human IGFALS capture antibody. The detection antibody is a biotinylated monoclonal mouse anti-human IGFALS antibody. This kit is specifically designed for the accurate quantitation of human IGFALS from cell culture supernatant, serum, plasma and other biological fluids. Free or ternary complex IGFALS can be detected. It is analytically validated with ready-to-use reagents.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Anti-Human IGFALS Pre-coated 96-well Strip Microplate
  • Human IGFALS Dectection Antibody
  • Human IGFALS Standard
  • Avidin-HRP
  • Assay Buffer B
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
Human
Application

ELISA

Sensitivity
4.39 ± 0.57 ng/mL
Standard Range
15 - 480 ng/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Biology Area
Angiogenesis, Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability
Gene ID
3483 View all products for this Gene ID
UniProt
View information about IGFALS on UniProt.org

Related FAQs

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

Go To Top Version: 1    Revision Date: 10.24.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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