Ultra-LEAF™ Purified anti-mouse CXCL5 (LIX) Antibody

Pricing & Availability
Clone
A20098E (See other available formats)
Regulatory Status
RUO
Other Names
LPS-induced CXC chemokine (LIX), granulocyte chemotactic protein-2 (GCP-2), alveolar macrophage chemotactic factor-II (AMCF-II), epithelial neutrophil-activating protein (ENA78)
Isotype
Rat IgG2b, λ
Ave. Rating
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Product Citations
publications
A20098E_ULEAF_CXCL5_Antibody_031422
Recombinant mouse CXCL5 (LIX) (Cat. No. 573302) (black circles) induces the chemotaxis of mouse Baf3-mCXCR2 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-mouse CXCL5 (LIX) (clone A20098E) (purple squares) neutralizes recombinant mouse CXCL5 (LIX)-induced chemotaxis at 0.8 ng/mL on mouse Baf3-mCXCR2 cells in a dose-dependent manner, whereas the LEAF™ purified rat IgG2b, λ isotype control (gray triangles) does not have the effect. ND50 range: 0.025 - 0.25 µg/mL
  • A20098E_ULEAF_CXCL5_Antibody_031422
    Recombinant mouse CXCL5 (LIX) (Cat. No. 573302) (black circles) induces the chemotaxis of mouse Baf3-mCXCR2 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-mouse CXCL5 (LIX) (clone A20098E) (purple squares) neutralizes recombinant mouse CXCL5 (LIX)-induced chemotaxis at 0.8 ng/mL on mouse Baf3-mCXCR2 cells in a dose-dependent manner, whereas the LEAF™ purified rat IgG2b, λ isotype control (gray triangles) does not have the effect. ND50 range: 0.025 - 0.25 µg/mL
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662053 100 µg 287€
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662054 1 mg 964€
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Description

CXCL5 is an ELR-CXC chemokine characterized by a Glu-Leu-Arg (ELR) motif preceding the characteristic CXC sequence. CXCL5 chemoattracts and activates neutrophils during inflammation through the binding to CXCR2. CXCL5 exists in different NH2- and COOH-truncated forms, and both NH2-terminal and COOH-terminal truncation result in an increase in neutrophil chemotactic potency.  CXCL5 (8–78) and CXCL5 (9–78) induce a higher neutrophil influx in vivo compared with CXCL5 (1–78). CXCL5 regulates the availability of binding sites for other ELR-CXC chemokines released during inflammation, through its interaction with erythrocyte duffy antigen receptor (DARC). As a result, CXCL5 induces the increase of CXCL1 and CXCL2 in a model of pulmonary inflammation. Therefore, CXCL5 acts as a regulator of chemokine scavenging and pulmonary host defense to bacterial infection. Lung epithelial cells express CXCR2. Consequently, CXCL5 may modulate neutrophil transepithelial and transendothelial migration. Mouse CXCL5 is the ortholog of human CXCL6.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CXCL5 (LIX)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the chemotaxis induced by recombinant mouse CXCL5 (LIX) (Cat. No. 573302) at 0.8 ng/mL on mouse Baf3- mCXCR2 transfectant chemoattractant cells. ND50 range: 0.025 - 0.25 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2910492 (BioLegend Cat. No. 662053)

Antigen Details

Structure
Chemokine
Distribution

Endothelial cells, fibroblasts, monocytes, macrophages, platelets, alveolar type II cells

Function
CXCL5 chemoattracts and activates neutrophils during inflammation, and it is induced by LPS and inflammatory cytokines. NH2-terminal truncation enhances, whereas citrullination decreases the chemotactic properties of CXCL5.
Interaction
Neutrophils
Ligand/Receptor
CXCR2
Antigen References
  1. Wuyts A, et al. 1999. J Immunol. 163:6155-63.
  2. Jeyaseelan S, et al. 2005. Am J Respir Cell Mol Biol. 32:531-9.
  3. Mei J, et al. 2010. Immunity. 33:106-17.
  4. Montier A, et al. 2010. J Biol Chem. 285:29750-9.
  5. Grommes J and Soehnlein O. 2011. Mol Med. 17:293-307.
Gene ID
20304 View all products for this Gene ID
UniProt
View information about CXCL5 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 03.11.2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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  • Ultra-LEAF™ Purified anti-mouse CXCL5 (LIX)

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