Ultra-LEAF™ Purified anti-CCL15 Antibody

Pricing & Availability
Clone
A15015A (See other available formats)
Other Names
Macrophage inflammatory protein 1 (MIP1ž„), Macrophage inflammatory protein 5 (MIP5), Leukotactin 1 (LKN1), Hemofiltrate chemokine 2 (HCC-2), New CC chemokine 3 (NCC3)
Isotype
Mouse IgG2a, κ
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Product Citations
publications
A15015A_ULEAF_PURE_CCL15_Antibody_FA_111116
Recombinant human CCL15 chemoattracts Baf3-hCCR1 transfectants in a dose dependent manner (purple circles). Anti-human CCL15 antibody (clone A15015A, purple squares) neutralizes the chemoattraction of Baf3-hCCR1 transfectants induced by recombinant human CCL15 (6 ng/ml). The ND50 = 0.05 - 0.25 µg/ml.
  • A15015A_ULEAF_PURE_CCL15_Antibody_FA_111116
    Recombinant human CCL15 chemoattracts Baf3-hCCR1 transfectants in a dose dependent manner (purple circles). Anti-human CCL15 antibody (clone A15015A, purple squares) neutralizes the chemoattraction of Baf3-hCCR1 transfectants induced by recombinant human CCL15 (6 ng/ml). The ND50 = 0.05 - 0.25 µg/ml.
Cat # Size Price Quantity Avail. Save
692703 100 µg 196€
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692704 1 mg 520€
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Description

CCL15 was discovered by analyzing the upstream region of the CCL14 gene. CCL15 is arranged in tandem with CCL14 on human chromosome 17. CCL15, CCL6, CCL9, and CCL23 have an extended N-terminal domain upstream of the first two conserved chemokine cysteines. This domain is encoded by a different exon and includes 16 to 20 residues. CCL15 (1-92) is processed by cathepsin G to produce highly active forms of CCL15 [(24-92), (27-92) and (29-92)], and elastase processes CCL15 (1-92) predominantly to CCL15 (22-92). Also, CCL15 is cleaved by MT6-MMP/MMP25 to produce CCL15 with very high activity. MMP25 is localized primarily in neutrophil gelatinase granules, and it is released by neutrophil activation and degranulation in inflammatory reactions. High levels of CCL15 were detected in human renal cell carcinoma bone metastasis, and in vitro studies showed that CCL15 stimulates chemotaxis of osteoclast progenitors and enhances their differentiation in response to RANKL. CCL15 is abundant in bronchoalveolar lavage of patients with pulmonary sarcoidosis. Also, it is elevated in the serum of patients with severe asthma, and it is reduced by omalizumab, a humanized anti-IgE antibody that has been use in the treatment of asthma. It has been suggested that airway smooth muscle cells and basophils produce CCL15, and it might have an important role in asthma severity and persistence. In addition, high levels of CCL15 have been detected in the serum of patients with hepatocellular carcinoma, and human hepatic carcinoma cells express CCR1 and CCL15.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CCL15.
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by blocking. The ND50 is 0.05 - 0.25 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2632767 (BioLegend Cat. No. 692703)
AB_2632768 (BioLegend Cat. No. 692704)

Antigen Details

Distribution
CCL15 is produced by smooth muscle cells, basophils, and hepatic carcinoma cells.
Function
CCL15 induces migration of monocytes, eosinophils, and lymphocytes. CCL15 is activated by cathepsin G, elastase, and MMPs. TNFα and IFNγ synergistically induce CCL15 in human airway smooth muscle cells in culture.
Interaction
Monocytes, eosinophils, airway smooth muscle cells, hepatic carcinoma cells, human osteogenic sarcoma cells, and osteoclast progenitors.
Ligand/Receptor
CCR1, CCR3.
Cell Type
Basophils
Biology Area
Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Pardigol A, et al. 1998. Proc. Natl. Acad. Sci. USA 95:6308.
2. Richter R, et al. 2005. J. Immunol. 175:1599.
3. Berahovich RD, et al. 2005. J. Immunol. 174:7341.
4. Shimizu Y, et al. 2011. J. Biol. Regul. Homeost. Agents 25:177.
5. Starr AE, et al. 2012. J. Biol. Chem. 287:13382.
6. Weber KL, et al. 2012. PLoS One 7:e40799.
7. Li Y, et al. 2013. Br. J. Cancer 108:99.

Gene ID
6359 View all products for this Gene ID
UniProt
View information about CCL15 on UniProt.org

Related FAQs

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 3    Revision Date: 01.19.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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