- Regulatory Status
- Other Names
- EFNA1, EPLG1, TNFAIP4, LERK1, EFL1, B61
- Ave. Rating
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- Product Citations
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Ephrins are cell surface molecules with a wide range of biological functions that are involved in both embryogenesis and adult life. This includes important roles in boundary formation, cell migration, repulsive axon guidance, and the regulation of neuronal growth cone development. The ephrin family consists of eight members. Ephrin-A1 through A5 are anchored to the cell membrane via GPI linkage, while ephrin-B1 through B3 are transmembrane proteins. The interaction between ephrin ligands and its cognate Eph receptors involve both forward and reverse signaling.
Ephrin-A1 was originally isolated in conditioned media from HUVEC cells treated with TNF-α, and the gene was found to be induced by TNF-α in these cells. Ephrin-A1 is broadly expressed on endothelial cells and epithelial cells, but it can also be released as a soluble molecule. Ephrin-A1 and its primary receptor EphA2 play a vital role in normal angiogenesis and tumor neovascularization. Ephrin-A1 expressed on tumor cells inhibits tumor cell proliferation and migration, but also supports tumor growth by promoting angiogenesis. Ephrin-A1 is also expressed on resting CD4+ T cells and its expression is down regulated in asthma patients.
- Mouse Ephrin-A1, amino acids (Asp19-Ser182) (Accession# P52793), with human IgGFc and 6-His tag was expressed in 293E cells.
- Molecular Mass
- The 404 amino acid recombinant protein has a predicted molecular mass of approximately 46.6 kD. The protein migrates at approximately 60 kD in DTT reducing conditions and at approximately 120 kD in non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Asp.
- >95%, as determined by Coomassie stained SDS-PAGE.
- 0.22 µm filtered protein solution is in PBS, pH 7.2.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.) Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- EC50 = 3 - 12 ng/mL as measured by its ability to bind immobilized human EphA2 in a functional ELISA.
- Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at email@example.com.
- Monomer and Homodimer.
Endothelial cells, epithelial cells, resting CD4+ T cells.
- Regulation of angiogenesis, axongenesis, endothelial cell migration, integrin mediated adhesion, notochord formation, and negative regulation of epithelial to mesenchymal transition.
- Endothelial cells and epithelial cells.
- EphA2, EphA3, EphA4, EphA5, EphA6, and EphA7.
- Measured by its ability to bind immobilized human EphA2 in a functional ELISA.
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Antigen References
1. Wilkinson DG. 2001. Nat. Rev. Neurosci. 2:155-164.
2. Shamah SM, et al. 2001. Cell 105:233-244.
3. Holzman LB, et al. 1990. Mol. Cell Biol. 10:5830-5838.
4. Bartley TD, et al. 1994. Nature 368:558-560.
5. Brantley-Sieders DM, et al. 2006. Cancer Res. 66:10315-24.
6. Wohlfahrt JG, et al. 2004. J. Immunology 172:843-850.
7. Dai D, et al. 2014. Biochem. Biophys. Acta. 10:1729-40.
- Gene ID
- 13636 View all products for this Gene ID
- View information about Ephrin-A1 on UniProt.org
- Why choose BioLegend recombinant proteins?
• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins.
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)