Recombinant Mouse CXCL1 (KC) (carrier-free)

Pricing & Availability
Other Names
KC, Secreted protein N51, FSP, Grow regulated oncogen 1 (GRO1), Grow regulated oncogen a (GRO a), Melanoma growth stimulating activity (MGSA), MGSA-a, neutrophil activating protein 3 (NAP-3), SCYB1
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publications
CXCL1_Mouse_120811
Human neutrophils chemoattracted by mouse CXCL1.
  • CXCL1_Mouse_120811
    Human neutrophils chemoattracted by mouse CXCL1.
Cat # Size Price Quantity Avail. Save
573708 500 µg 2 080€
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573702 10 µg 120€
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573704 25 µg 260€
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573706 100 µg 800€
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Description

The CXCL1/KC gene was initially identified as being regulated by PDGF in BALB/c-3T3 cells. CXCL1/KC is an ELR CXC chemokine, and it is structurally and functionally related to the human GROs (CXCL1-CXCL3) and interleukin-8 (CXCL8).  CXCL1, CXCL2, and CXCL3 bind to the CXCR2 receptor, with CXCL1 having the highest affinity. CXCL1 is crucial for the recruitment of neutrophils to inflammatory sites.  In addition, CXCL1 has pleiotropic effects on cell proliferation, tumor angiogenesis, invasion, and metastasis. CXCL1 and CXCL2 induce neutrophil mobilization from the bone marrow in a murine model of acute peritonitis, and CXCL1 and G-CSF act cooperatively in this process. In addition, CXCL1 mediates murine sickle cell vasoocclusion during hemolytic transfusion reactions. Thrombin enhances the secretion of CXCL1 from tumor cells, and PGE2 stimulates expression of CXCL1 in human colorectal carcinoma cells (CRC). Furthermore, CXCL1 (secreted by CRC cells) induces endothelial cell migration and tube formation in vitro and in vivo. CXCL1 is induced by inflammatory cytokines such as IL-1 and TNF, and IL-17A enhances expression of CXCL1 by prolonging the half-life of its constitutively unstable mRNA.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Mouse CXCL1, amino acids Asn29-Lys96 (Accession# NM_008176.3) was expressed in E. coli.
Molecular Mass
The 68 amino acid recombinant protein has a predicted molecular mass of approximately 7.4 kD. The DTT-reduced protein migrates at approximately 10 kD and non-reduced protein migrates at slightly higher than 10 kD by SDS-PAGE. The N-terminal amino acid is Asparagine.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Bioactivity was measured by its property to chemoattract human neutrophils in a dose dependent manner.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Product Citations
  1. Kamp M, et al. 2016. PLoS One. 11: 0163750. PubMed
  2. Kumar V, et al. 2017. Cancer Cell. 32:654. PubMed
  3. Shin JW, et al. 2020. Cell Death Dis. 11:301. PubMed

Antigen Details

Structure
Chemokine
Distribution
CXCL1 is produced by neutrophils, activated monocytes, macrophages, fibroblasts, keratinocytes, epithelial cells, and endothelial cells.
Function
CXCL1 chemoattracts neutrophils, and induces cell proliferation, tumor angiogenesis, invasion, and metastasis. CXCL1 is induced by IL-1, TNF, thrombin, and PGE2.
Interaction
Neutrophils and endothelial cells
Ligand/Receptor
CXCR2
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Cochran BH, et al. 1983. Cell 33:939.
2. Anisowicz A, et al. 1987. P. Natl. Acad. Sci. USA 84:7188.
3. Caunt M, et al. 2006. Cancer Res. 66:4125.
4. Wang D, et al. 2006. J. Exp. Med. 203:941.
5. Wengner AM, et al. 2008. Blood 111:42.
6. Stillie R, et al. 2009. J. Leukoc. Biol. 86:529.
7. Datta S, et al. 2010. J. Immunol. 184:1484.
8. Jang JE, et al. 2011. J. Clin. Invest. 121:1397.

Gene ID
14825 View all products for this Gene ID
UniProt
View information about CXCL1 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 2    Revision Date: 08.19.2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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