Recombinant Human u-Plasminogen Activator (Urokinase) (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
uPA, PLAU, ATF, QPD, UPA, URK, u-PA, BDPLT5
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Product Citations
publications
u-PlasminogenActivator-Urokinase_Human_RECOM_BA_022316
The activity of uPA was measured with 100 µM of fluorogenic uPA substrate, Bz-GGR-AMC, in the presence of 0.625, 1.25, 2.5, 5, 10, 20, and 40 ng of human uPA.
  • u-PlasminogenActivator-Urokinase_Human_RECOM_BA_022316
    The activity of uPA was measured with 100 µM of fluorogenic uPA substrate, Bz-GGR-AMC, in the presence of 0.625, 1.25, 2.5, 5, 10, 20, and 40 ng of human uPA.
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755302 10 µg 148,00€
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755304 25 µg 260,00€
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Description

Urokinase-type plasminogen activator, also known as uPA, is secreted as a 411 amino acid single-chain proenzyme that is composed of an N-terminal GFD (growth factor-like domain) (amino acids 1–43), which binds to uPAR, a kringle domain (amino acids 44–135) and a C-terminal serine protease domain (amino acids 136–411). The proenzyme has little or no enzymatic activity, and it is activated upon cleavage by plasmin at Lys158-Iso159 to generate a two-chain molecule, which converts plasminogen into plasmin. The activated enzyme (uPA) cleaves other substrates such as VEGF and HGF. Sequentially, plasmin degrades the ECM and stimulates the local release of growth factors promoting cell adhesion, migration, and proliferation. The receptor for uPA is present on the surface of many cell types, it regulates proteolysis by binding the extracellular protease uPA, and also activates many intracellular signaling pathways that promote cell motility, invasion, proliferation, and survival. Thus, uPAR interaction with β1 integrin is required for PDGF-AB-induced human mesenchymal stem (MSC) and stromal cell migration. Additional studies have shown that uPA and PDGF have a synergistic effect on airway smooth muscle cell migration. uPA/uPAR also play a key role in regulating the migration and differentiation of MSC. uPAR is overexpressed in a variety of tumor cell lines and tissues, which include breast, ovary, lung, pancreas, colon, kidney, liver, stomach, and the endometrium. High levels of uPAR are associated with cancer invasion and metastasis.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human uPA, amino acids (Ser21-Leu431) (Accession# P00749.2), with C-terminal TG-10His-GGQ tag was expressed in 293E cells.
Molecular Mass
The 426 amino acid recombinant protein has a predicted molecular mass of approximately 48.1 kD. The DTT-reduced protein migrates at approximately 18 kD (chain A) and 38 kD (chain B) and the non-reduced protein migrates at approximately 55 kD by SDS-PAGE.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 1.0 EU per µg of protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL.
Storage & Handling
Unopened vial can be stored at -70°C for six months. For maximum results, quick spin vial prior to opening. Avoid repeated freeze/thaw cycles.
Activity
Human uPA cleaves the peptide substrate Bz-GGR-AMC with a specific activity value higher than 2,000 pmol/µg/min.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Monomer as proenzyme and dimer activated enzyme.
Distribution
Urine, blood, and extracelluar matrix.
Function
Converts plasminogen into plasmin, cell migration, angiogenesis, wound repair, inflammation, immunity, and tumor metatases. uPA mediates neointimaformation after injuries.
Interaction
Many cell types such as myeloid cells, endothelial cells, epithelial cells, vascular smooth muscle cells, and mesenchymal stem cells.
Ligand/Receptor
uPA receptor (uPAR, CD87).
Bioactivity
Human uPA activity is measured by its ability to cleave a fluorogenic peptide substrate Bz-GGR-AMC.
Biology Area
Stem Cells
Molecular Family
Enzymes and Regulators
Antigen References

1. Lijnen HR, et al. 1988. Eur. J. Biochem. 172:185.
2. Ploug M, et al. 1991. J. Biol. Chem. 266:1926.
3. Carlin SM, et al. 2003. Am. J. Physiol. Lung Cell Mol. Physiol. 284:L1020.
4. Gutova M, et al. 2008. Stem Cells 26:1406.
5. Smith HW, Marshal CJ. 2010. Nature Rev. Mol. Cell Biol. 11:23.
6. Li D, et al. 2013. Theranostics 3:507.
7. Chabot V, et al. 2015. Stem Cell Res. Ther. 6:188.

Gene ID
5328 View all products for this Gene ID
UniProt
View information about u-Plasminogen Activator on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
Go To Top Version: 1    Revision Date: 02.24.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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