Recombinant Human SAA1 (carrier-free)

Pricing & Availability
Regulatory Status
Other Names
Serum amyloid A1
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Product Citations
Human SAA1 induces IL-6 in neonate human skin fibroblasts.
  • SAA1_CF_Human_RECOM_BA_033016
    Human SAA1 induces IL-6 in neonate human skin fibroblasts.
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756404 25 µg 100,00€
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756406 100 µg 220,00€
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The serum amyloid A family of proteins is encoded by multiple genes, and it shows a high degree of homology in mammals. The human genome includes four SAA genes: SAA1 and SAA2, which are acute-phase proteins, SAA3 is a pseudogene (while the murine homolog codes for SAA3 protein), and SAA4 is a constitutively expressed protein. SAA1 is a non-glycosylated acute-phase protein, which can increase up to 1000-fold during inflammation. High levels of SAA1 and SAA2 correlate with the severity of inflammatory diseases such as atherosclerosis, rheumatoid arthritis, and cancer. SAA binds to the G protein-coupled formyl peptide receptor 2 (FPR2). Additionally, SAA stimulates G-CSF production through TLR2, leading to neutrophilia. Also, SAA binding to TLR2 results in NF-κB activation and enhanced expression of proinflammatory and anti-apoptotic genes. Thus SAA contributes to the inflammatory process. SAA1 in plasma is associated with high density lipoproteins (HDL), and modulates the atheroprotective properties of HDL during inflammation.

Product Details
Technical Data Sheet (pdf)

Product Details

Human SAA1, amino acids Met-(Arg19-Tyr122) (Accession# NM_001178006), was expressed in E. coli.
Molecular Mass
The 105 amino acid recombinant protein has a predicted molecular mass of approximately 11.8 kD. The protein migrates at approximately 13 kD in both DTT-reducing conditions and non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Met.
>95%, as determined by Coomassie stained SDS-PAGE.
0.22 µm filtered protein solution is in 10% Glycerol and TBS in pH 8.0.
Endotoxin Level
Less than 0.1 EU per µg of protein as determined by the LAL method.
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Human SAA1 induces IL-6 in neonate human skin fibroblast with an ED50 = 2.5 - 12.5 µg/mL.


Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at

Product Citations

Antigen Details



Cholesterol transport, HDL metabolism, and host defense. Induced by pro-inflammatory cytokines and is downregulated by IL-1 receptor antagonist.
Rheumatoid synoviocytes, intestinal epithelial cells, monocytes, macrophages, and neutrophils.
Heparin, HDL formyl peptide receptor 2 (FPR2), and Toll-like receptor 2 (TLR2).
Biology Area
Immunology, Innate Immunity
Antigen References

1. Jensen LE, Whitehead AS, 1998. Biochem. J. 334:489.
2. Artl A, et al. 2000. Arterioscler Thromb Vasc Biol. 3:763-72.
3. Lee MS, et al. 2006. J. Immunol. 177:5585.
4. He RL, et al. 2009. Blood 113:429.
5. Patke S, et al. 2013. PLoS One 6:e64974.
6. Rossmann C, et al. 2014. Biochem. Biophys. Res. Commun. 450:1643.
7. Chen M, et al. 2014. Immunobiology 12:916-23.

Gene ID
6288 View all products for this Gene ID
View information about SAA1 on

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
Go To Top Version: 1    Revision Date: 03.31.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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