Purified anti-RRM2 Antibody

Pricing & Availability
Clone
W19033A (See other available formats)
Regulatory Status
RUO
Other Names
ribonucletide-diphosphate reductase subunit M2, ribonucleotide reductase small subunit, RRM2, RNR2
Isotype
Rat IgG2b, κ
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Product Citations
publications
W19033A_PURE_RRM2_Antibody_1_081920
Whole cell extracts (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-RRM2 antibody (clone W19033A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W19033A_PURE_RRM2_Antibody_1_081920
    Whole cell extracts (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-RRM2 antibody (clone W19033A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W19033A_PURE_RRM2_Antibody_2_081920
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.0 µg of purified rat IgG2b, κ isotype ctrl antibody (Cat. No. 400601) or purified anti-RRM2 antibody (clone W19033A). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with a rabbit control antibody against a separate epitope of RRM2. Lane M: Molecular weight marker.
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937401 25 µg 68,00€
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937402 100 µg 172,00€
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Description

Ribonucleotide reductase catalyzes the rate-limiting step for de novo deoxyribonucleotide dNTPs synthesis. It is a heterotetramer comprised of two identical large subunits (RRM1) and two identical small subunits (RRM2). The functional ribonucleotide reductase enzyme converts ribonucleotides to dNTPs, which are utilized for DNA synthesis. Ribonucleotide reductase activity requires a high degree of regulation to maintain a proper balance between dNTP availability and DNA synthesis demands. While RRM1 protein levels are generally constitutive, RRM2 expression is cell-cycle regulated at multiple levels. Dysregulation of RRM2 results in dNTP imbalances and increased misincorporation of dNTPs into newly synthesized DNA, and elevated RRM2 levels have been described in multiple forms of cancer. Targeted disruption of RRM2 has been proposed as a mechanism to drive tumors into a senescent state.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full-length recombinant human RRM2 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not recognize mouse RRM2 by western blot.

This clone was tested for ICC using HeLa cells fixed with 4% PFA and permeabilized with Triton X-100 or methanol; RRM2 staining failed under both conditions.

RRID
AB_2876752 (BioLegend Cat. No. 937401)
AB_2876752 (BioLegend Cat. No. 937402)

Antigen Details

Structure
RRM2 is a 389 amino acid protein with a predicted molecular weight of 45 kD.
Distribution

Cytosol/Ubiquitously expressed

Function
DNA synthesis
Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication
Molecular Family
Enzymes and Regulators
Antigen References
  1. Zhang K, et al. 2009. Mol Cancer. 8:11.
  2. Zhang YW, et al. 2009. J Biol Chem. 284:18085-95.
  3. D’Angiolella V, et al. 2012. Cell. 149:1023-34.
Gene ID
6241 View all products for this Gene ID
UniProt
View information about RRM2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08.19.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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