- W18205A (See other available formats)
- Regulatory Status
- Other Names
- Protein Kinase C iota, Atypical Protein Kinase C-lambda/iota, Protein Kinase C iota Type, PRKC-lambda/iota, aPKC-lambda/iota, atypical protein kinase C, aPKC, aPKCλ/ι
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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Members of the protein kinase c family (PKC) are serine/threonine protein kinases with a broad set of substrates that can regulate diverse cellular processes. PKCι, also referred to as PKCι/λ, is a member of the atypical subfamily of PKC, which is characterized by complete independence of calcium and diacylglycerol as second messengers. It has been shown to play a role in cancer survival through multiple mechanisms: a. phosphorylation of the DNA-binding domain of FoxO1 resulting in transcriptional reprogramming of c-myc, which leads to increased proliferation of angiosarcoma cells, b. regulating membrane trafficking of type 1-matrix metalloprotease from late endosomal compartments to the plasma membrane, and c. mediating pro-survival by phosphorylating and inhibiting the pro-apoptotic factor BAD in glioblastomas. PKCι is also required for resistance to anti-apoptotic drugs in BCR-Abl-dependent leukemia and may represent a viable target for combinatorial therapies.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- Partial recombinant human PKCι protein.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
IP - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone was tested for ICC using HeLa cells fixed with 4% PFA and permeabilized with methanol or Triton X-100; it failed due to weak staining.
- Product Citations
AB_2820114 (BioLegend Cat. No. 671201)
AB_2820115 (BioLegend Cat. No. 671202)
- PKCι is a 596 amino acid protein with a predicted molecular weight of 68 kD.
Cytosol and Nucleus/Ubiquitously expressed
- Protein Kinase/Cell Signaling
- Biology Area
- Cell Biology
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
- Nayak RC, et al. 2019. Nat. Commun. 10:46.
- Riddell M, et al. 2018. Nat. Commun. 9:5357.
- Rosse C, et al. 2014. Proc Natl Acad Sci U S A. 111:E1872.
- Desai S, et al. 2011. Biochim Biophys Acta. 1813:1190.
- Ishiguro H, et al. 2009. Proc Natl Acad Sci U S A. 106:16369.
- Gene ID
- 5584 View all products for this Gene ID
- View information about PKCiota on UniProt.org
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