Purified anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
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Product Citations
publications
30-F11_Pure_071608.jpg
BALB/c splenocytes stained with 30-F11 purified, followed by anti-rat IgG FITC
  • 30-F11_Pure_071608.jpg
    BALB/c splenocytes stained with 30-F11 purified, followed by anti-rat IgG FITC
  • 30-F11_Pure_CD45_Antibody_2_102518
    Fresh, frozen mouse spleen was stained with purified CD45 clone 30-F11 conjugated and detected with a FITC CODEX™ oligonucleotide duplex (red). Samples were counterstained with CollagenIV Cy5 (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
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103101 50 µg 24,00€
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103102 500 µg 76,00€
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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, CyTOF® - Verified
IP, CMCD, IHC, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6 and Western blotting7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
Product Citations
  1. Huppé CA, et al. 2018. Mucosal Immunol. 0.536111111. PubMed
  2. Davies M, et al. 2017. PLoS Pathogens. 13(6):e1006435. PubMed
  3. Cédile O, Wlodarczyk A, and Owens T 2017. APMIS.. 10.1111/apm.12740. PubMed
  4. Peralta Ramos JM, et al. 2017. Front Immunol. 1.490277778. PubMed
  5. Sutton C, et al. 2017. Nat Commun.. 10.1038/s41467-017-02111-0. PubMed
  6. Seehus CR, et al. 2017. Nat Commun. 8:1900. PubMed
  7. Rhee S, et al. 2018. Nat Commun. 9:368. PubMed
  8. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  9. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  10. Jones KB et al. 2018. Cell stem cell. 24(1):183-192 . PubMed
  11. Murai K et al. 2018. Cell stem cell. 23(5):687-699 . PubMed
  12. Moon H et al. 2017. Cell stem cell. 21(5):665-678 . PubMed
  13. Starossom SC, et al. 2019. Nat Commun. 10:217. PubMed
  14. Zabaleta N, et al. 2018. Nat Commun. 9:5454. PubMed
  15. Gao J, et al. 2017. Oncol Lett. 14:2954. PubMed
  16. Baluk P, et al. 2018. Methods Mol Biol. 1846:161. PubMed
  17. Emrick JJ, et al. 2018. Proc Natl Acad Sci U S A. 115:E12091. PubMed
  18. Krenzlin H, et al. 2019. J Clin Invest. 130:1671. PubMed
  19. Finn J et al. 2019. Cell reports. 26(11):2942-2954 . PubMed
  20. Stokes KL, et al. 2019. Oncogenesis. 8:24. PubMed
  21. Vu LT, et al. 2019. J Extracell Vesicles. 8:1599680. PubMed
  22. Deng W et al. 2019. Cell reports. 27(6):1755-1768 . PubMed
  23. Moon H, et al. 2019. Nat Commun. 10:2225. PubMed
  24. Thulasingam S, et al. 2019. Int J Mol Sci. 20. PubMed
  25. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  26. Axelrod HD, et al. 2019. Mol Cancer Res. 17:356. PubMed
  27. Bernard S, et al. 2018. J Mammary Gland Biol Neoplasia. 23:249. PubMed
  28. Haber AL, et al. 2017. Nature. 551:333. PubMed
  29. Schneider H, et al. 2017. J Neurochem. 140:170. PubMed
  30. Widjaja AA, et al. 2019. Gastroenterology. 157:777. PubMed
  31. Zhu F, et al. 2019. J Immunol. 203:2644. PubMed
  32. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  33. Li Y et al. 2019. Endocrinology. 160(4):938-946 . PubMed
  34. Morita S et al. 2017. Cell metabolism. 25(4):883-897 . PubMed
  35. He X, et al. 2017. Cancer Biol Ther. 0.815277778. PubMed
  36. Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
  37. Shafer MER, et al. 2017. Stem Cell Reports. 1.040277778. PubMed
  38. Ajona D, et al. 2017. Cancer Discov. 0.773611111. PubMed
  39. Fino KK, et al. 2017. Sci Rep. 5.334722222. PubMed
  40. Goel S, et al. 2017. Nature. 548:471. PubMed
  41. Poulos MG, et al. 2017. J Clin Invest. 127:4163. PubMed
  42. Nolin JD, et al. 2017. JCI Insight. 2:e94929. PubMed
  43. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
  44. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  45. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
  46. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  47. Sheppard S, et al. 2018. Front Immunol. 1.630555556. PubMed
  48. McLeod RL, et al. 2018. Oncotarget. 9:34459. PubMed
  49. Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
  50. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  51. Leist SR, et al. 2019. PLoS One. 14:e0220126. PubMed
  52. Rothchild AC, et al. 2019. Sci Immunol. 4:eaaw6693. PubMed
  53. Fiege JK, et al. 2019. PLoS Pathog. 15:e1008077. PubMed
  54. Langer V, et al. 2019. J Clin Invest. 129:4691. PubMed
  55. Wolf Y, et al. 2017. J Exp Med. 214:905. PubMed
  56. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  57. Chao Y, et al. 2020. Sci Adv. 6:eaaz4204. PubMed
  58. Davies CL, et al. 2019. Front Immunol. 10:1048. PubMed
  59. Chen X, et al. 2019. Methods Mol Biol. 2048:131. PubMed
  60. Lee YJ, et al. 2018. Front Microbiol. 9:83. PubMed
  61. Zhang J, et al. 2019. Onco Targets Ther. 12:4985. PubMed
  62. Shao Y, et al. 2017. Onco Targets Ther. 10:2675. PubMed
  63. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  64. Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed
  65. Hitchcock JR, et al. 2020. FEBS J. 287:250. PubMed
  66. Silvestre-Roig C, et al. 2019. Nature. 569:236. PubMed
  67. Seelige R, et al. 2018. Sci Rep. 8:13670. PubMed
  68. Ito S, et al. 2020. PLoS One. 15:e0229888. PubMed
  69. Qiu X, et al. 2018. Lab Chip. 18:2776. PubMed
  70. Paharik AE, et al. 2017. Cell Host Microbe. 22:746. PubMed
  71. Bar O, et al. 2020. Fluids Barriers CNS. 17:27. PubMed
  72. Mensurado S, et al. 2018. PLoS Biol. 16:e2004990. PubMed
  73. Kim D, et al. 2019. Immune Netw. 19:e32. PubMed
  74. Tamburini BAJ, et al. 2019. Front Immunol. 10:1036. PubMed
  75. Rincón E, et al. 2017. Oncotarget. 8:45415. PubMed
  76. Heinrich A, et al. 2020. Cell Rep. 31:107513. PubMed
  77. Vitiello GA, et al. 2018. Clin Cancer Res. 24:972. PubMed
  78. Paulo E, et al. 2018. Sci Rep. 8:11001. PubMed
  79. Lee S, et al. 2020. Cell Host & Microbe. 27(6):937-949. PubMed
  80. Melchor SJ, et al. 2020. Sci Rep. 10:15724. PubMed
  81. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  82. Murad JP, et al. 2018. Front Immunol. 1.95. PubMed
  83. Matsuo K, et al. 2018. J Invest Dermatol. 138:1764. PubMed
  84. Podd B, et al. 2006. J Immunol. 176:6532. PubMed
  85. Latasa M, et al. 2010. PLoS One. 5:e15690. PubMed
  86. DeFalco T, et al. 2014. Proc Natl Acad Sci U S A. 111:2384. PubMed
  87. Nikolaou K, et al. 2014. EMBO J. 34:430. PubMed
  88. Cha J, et al. 2015. J Biol Chem. 290:15337. PubMed
  89. Gereke M, et al. 2012. J Vis Exp. 70: 4322. PubMed
  90. Zareie P, et al. 2017. J Neuroinflammation. 0.630555556. PubMed
  91. Hernández-Santana YE, et al. 2020. Life Sci Alliance. 3:00. PubMed
  92. Claveria-Cabello A, et al. 2020. Cancers (Basel). 12:00. PubMed
  93. Li X, et al. 2020. Cell Rep. 31:107765. PubMed
  94. Fu H, et al. 2020. Front Immunol. 11:595813. PubMed
  95. Li Y, et al. 2020. Elife. 9:00. PubMed
  96. Parlanti P, et al. 2020. Exp Eye Res. 194:107998. PubMed
  97. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  98. Karhadkar TR, et al. 2021. PLoS One. 16:e0245924. PubMed
  99. Chen Q, et al. 2021. STAR Protocols. 2(1):100241. PubMed
  100. Chamberlin T, et al. 2020. Cancer Research. 80(20):4465-4475. PubMed
  101. Mori Y, et al. 2021. Diabetes and Vascular Disease Research. 18(3):14791641211027324. PubMed
  102. Kenswil KJG, et al. 2021. Cell Stem Cell. 28(4):653-670.e11. PubMed
  103. Altshuler A, et al. 2021. Cell Stem Cell. 28(7):1248-1261.e8. PubMed
RRID
AB_312966 (BioLegend Cat. No. 103101)
AB_312967 (BioLegend Cat. No. 103102)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 10.25.2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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