Purified anti-mouse CD3ε Antibody

Pricing & Availability
Clone
145-2C11 (See other available formats)
Regulatory Status
RUO
Other Names
CD3ε, T3, CD3
Isotype
Armenian Hamster IgG
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Product Citations
publications
145-2C11_Pure_021606
C57BL/6 mouse splenocytes were stained with purified CD3e (clone 145-2C11) (filled histogram) or Armenian hamster IgG isotype control (open histogram), followed by anti-Armenian hamster IgG FITC.
  • 145-2C11_Pure_021606
    C57BL/6 mouse splenocytes were stained with purified CD3e (clone 145-2C11) (filled histogram) or Armenian hamster IgG isotype control (open histogram), followed by anti-Armenian hamster IgG FITC.
  • 145-2C11_PURE_CD3e_Antibody_IHC-F_033017
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD3ε (clone 145-2C11) and 10 µg/mL of Alexa Fluor® 488 anti-mouse/human CD45R/B220 (clone RA3-6B2) (green) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 594 Goat anti-hamster (Armenian) IgG (clone Poly4055) (red) for two hours at room temperature. The image was captured by 10X objective.
  • 145-2C11_PURE_CD3e_Antibody_IHC-F_2_102518
    Fresh, frozen mouse spleen was stained with purified CD3ε clone 145-2C11 conjugated and detected with a Cy3 CODEX™ oligonucleotide duplex (red). Samples were counterstained with B220 A647 (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
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100301 50 µg 18€
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100302 500 µg 58€
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Description

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The CD3ε antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IP, Activ, Block, WB, ICC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32,  and in vivo T cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block)
  2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
  3. Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
  4. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
  5. Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
  6. Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
  7. Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
  8. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
  9. Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
  10. Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
  11. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
  12. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
  13. Ko SY, et al. 2005. J. Immunol. 175:3309.
  14. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F)
  15. Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F)
  16. Wang W, et al. 2007. J. Immunol. 178:4885. (Activ)
  17. Xiao S, et al. 2007. J. Exp. Med. 204:1691.
  18. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed.
  19. Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed
  20. Guo Y, et al. 2008. Blood 112:480. PubMed
  21. Kenna TJ, et al. 2008. Blood 111:2091.
  22. Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed
  23. Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed
  24. Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed
  25. de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed
  26. Schulteis RD, et al. 2008. Blood 295:G273. PubMed
  27. Qi Q, et al. 2009. Blood 114:564. PubMed
  28. Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed
  29. Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed
  30. Yan J, et al. 2014. Vaccine. 32:2833. PubMed
  31. Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed
  32. Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
Product Citations
  1. Namavari A, et al. 2012. Invest Ophthalmol Vis Sci . 53:4575. PubMed
  2. Sandy A, et al. 2013. J Immunol. 190:5818. PubMed
  3. Wang G, et al. 2015. PLoS One. 10:121968. PubMed
  4. McCully M, et al. 2015. J Immunol. 195: 96 - 104. PubMed
  5. Bremser A, et al. 2015. PLoS One. 10: 0137393. PubMed
  6. Kim K, et al. 2016. PLoS One. 11: 0148576. PubMed
  7. Kojima T, et al. 2016. Sci Rep. 6:36457. PubMed
  8. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
  9. Hsieh WC, et al. 2020. JCI Insight. 5:00. PubMed
  10. Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
  11. Sharma M, et al. 2020. Circ Res. 127:335. PubMed
  12. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  13. Cain MP, et al. 2020. Dis Model Mech. 13:00. PubMed
  14. Takushi SE, et al. 2020. Hum Gene Ther. 31:626. PubMed
  15. Gaspar M, et al. 2020. Cancer Immunol Res. 0.875694444. PubMed
  16. Yi M, et al. 2021. J Hematol Oncol. 14:27. PubMed
  17. Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed
  18. Jennings EK, et al. 2021. STAR Protocols. 2(1):100284. PubMed
  19. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  20. McLane LM, et al. 2021. Cell Reports. 35(6):109120. PubMed
  21. Lu SX, et al. 2021. Cell. . PubMed
  22. Rissiek B, et al. 2017. Sci Rep. . 10.1038/s41598-017-16613-w. PubMed
  23. Lin J, et al. 2017. Nat Commun. . 10.1038/s41467-017-01477-5. PubMed
  24. Won HY, et al. 2017. Sci Rep. 7:16946. PubMed
  25. Coley WD et al. 2018. F1000Research. 2018 Mar 14 [revised 2018 Jan 1] 0.5125. PubMed
  26. Hao Y, et al. 2018. Front Immunol. 9:1127. PubMed
  27. Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
  28. Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed
  29. An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed
  30. Sekiya T et al. 2018. Cell reports. 24(6):1627-1638 . PubMed
  31. Nagatake T, et al. 2018. Int Immunol. 30:471. PubMed
  32. Baluk P, et al. 2018. Methods Mol Biol. 1846:161. PubMed
  33. Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed
  34. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  35. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  36. Niven J, et al. 2019. Cell Rep. 28:21. PubMed
  37. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  38. Akiyama C, et al. 2019. J Immunol. 203:835. PubMed
  39. Oghumu S, et al. 2019. J Immunol. 203:789. PubMed
  40. Lyon de Ana C, et al. 2019. J Immunol. 202:1112. PubMed
  41. Simmons S et al. 2019. Elife. 8 pii: e41239. PubMed
  42. Wu P et al. 2019. Molecular cell. 73(5):1015-1027 . PubMed
  43. Duc D et al. 2019. Cell Rep. 29(2):378-390 . PubMed
  44. Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
  45. Kälin S et al. 2017. Cell metabolism. 26(3):475-492 . PubMed
  46. Morabito KM, et al. 2017. Mucosal Immunol. 0.795138889. PubMed
  47. Parzmair GP, et al. 2017. J Leukoc Biol. 101:407. PubMed
  48. Arimura K, et al. 2017. Mucosal Immunol. 1.08125. PubMed
  49. Van De Velde LA, et al. 2017. J Biol Chem. 292:15:00. PubMed
  50. Tuzlak S, et al. 2017. Cell Death Differ. 24:523. PubMed
  51. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  52. Wang HC, et al. 2017. J Immunol. 198:3149. PubMed
  53. Bergfeld A, et al. 2017. Front Microbiol. 0.919444444. PubMed
  54. Scholz T, et al. 2017. PLoS One. 12:e0182646. PubMed
  55. Goel S, et al. 2017. Nature. 548:471. PubMed
  56. Link MA, et al. 2017. PLoS One. 12:e0184320. PubMed
  57. Abe Y, et al. 2017. Biochem Biophys Rep. 0.543055556. PubMed
  58. Zhou AC, et al. 2017. Front Immunol. 1.136111111. PubMed
  59. Cui X, et al. 2017. J Immunol. 199:4066. PubMed
  60. Wang W, et al. 2018. J Vasc Surg. 68:1538. PubMed
  61. Parigi SM, et al. 2018. Sci Rep. 0.440277778. PubMed
  62. Chartrand K, et al. 2018. Front Immunol. 1.642361111. PubMed
  63. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  64. Robert S, et al. 2018. Bone Marrow Transplant. 53:807. PubMed
  65. Sprouse ML, et al. 2018. JCI Insight. 3:e97322. PubMed
  66. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  67. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  68. Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed
  69. Kikuchi K, et al. 2018. J Immunol. 201:635. PubMed
  70. Crawford G, et al. 2018. Nat Immunol. 1.388194444. PubMed
  71. Biljes D, et al. 2017. EXCLI J. 0.86875. PubMed
  72. McLeod RL, et al. 2018. Oncotarget. 9:34459. PubMed
  73. Akkaya B, et al. 2018. J Immunol. 201:3294. PubMed
  74. Chemnitz J, et al. 2019. Sci Rep. 3.745138889. PubMed
  75. Sharabi A, et al. 2019. JCI Insight. 4:e126294. PubMed
  76. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  77. Gartlan KH, et al. 2019. Blood Adv. 2.110416667. PubMed
  78. Wong E, et al. 2019. Cell Rep. 29:3047. PubMed
  79. Hanoteau A, et al. 2019. J Immunother Cancer. 7:10. PubMed
  80. Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
  81. Borges TJ, et al. 2018. Nat Commun. 9:3482. PubMed
  82. Du J, et al. 2018. AMB Express. 8:158. PubMed
  83. Seo N, et al. 2018. Nat Commun. 9:435. PubMed
  84. Klein JC, et al. 2017. Nat Commun. 8:14600. PubMed
  85. Spranger S, et al. 2017. Cancer Cell. 31:711. PubMed
  86. Teijeira A, et al. 2018. Cancer Immunol Res. 6:798. PubMed
  87. Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed
  88. Afzali B, et al. 2017. Nat Immunol. 18:813. PubMed
  89. Cole C, et al. 2018. Nucleic Acids Res. 46:e62. PubMed
  90. Khameneh HJ, et al. 2017. J Immunol. 198:196. PubMed
  91. Fritz Y, et al. 2017. J Invest Dermatol. 137:696. PubMed
  92. Wolf Y, et al. 2017. J Exp Med. 214:905. PubMed
  93. Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed
  94. Field CS, et al. 2018. Oncoimmunology. 7:e1376154. PubMed
  95. Carpenter SM, et al. 2017. PLoS Pathog. 13:e1006704. PubMed
  96. Niss Arfelt K, et al. 2017. Blood. 129:866. PubMed
  97. Edenborough KM, et al. 2019. Front Immunol. 10:2414. PubMed
  98. Rao TN, et al. 2020. J Immunol. 204:2600. PubMed
  99. Sanctuary MR, et al. 2019. Mucosal Immunol. 0.638888889. PubMed
  100. Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed
  101. Cohen MA, et al. 2020. Cell Stem Cell. 26(4):579-592. PubMed
  102. Chen L, et al. 2019. Nat Commun. 3.553472222. PubMed
  103. Wang N, et al. 2020. Front Immunol. 1.765972222. PubMed
  104. Majer O, et al. 2019. Nature. 575:366. PubMed
  105. Genoud V, et al. 2018. Oncoimmunology. 7:e1501137. PubMed
  106. Perchonock C, et al. 2006. Mol Cell Biol . 26:6005. PubMed
  107. Perchonock C, et al. 2007. J Immunol. 179:1768. PubMed
  108. Guo Y, et al. 2008. Blood. 112:480. PubMed
RRID
AB_312666 (BioLegend Cat. No. 100301)
AB_312667 (BioLegend Cat. No. 100302)

Antigen Details

Structure
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
TCR signal transduction, T cell activation, antigen recognition
Ligand/Receptor
Peptide antigen/MHC-complex
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12501 View all products for this Gene ID
UniProt
View information about CD3epsilon on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 10.17.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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