Purified anti-human IL-32α Antibody

Pricing & Availability
KU32-07 (See other available formats)
Regulatory Status
Other Names
IL-32 alpha,, Interleukin 32 alpha, Natural Killer Transcript 4 (NK4), TNF-α-inducible factor (TAIFa)
Mouse IgG2b, κ
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Product Citations
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513301 50 µg 102€
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513302 500 µg 419€
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Interleukin 32 (IL-32), previously known as a transcript (NK4), is produced by mitogen-activated lymphocytes, by IFNγ-activated epithelial cells or by IL-12 and IL-18-activated NK cells. Its expression is increased following activation of T-cells by mitogens or the activation of NK cells by IL-2. IL-32 activates NF-κB and p38 MAPK cytokine signal pathways. It has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis. IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues. IL-32 exists in at least four differentially spliced isoforms (α, β, γ and δ) with predicted molecular weight: ~26 kD. IL-32α is the shortest and most abundant of four potential splice variants of the pro-inflammatory cytokine IL-32. Potential modifications include myristoylation and N-glycosylation. Transfected IL-32α was more likely to be cell-associated as compared to IL-32β, suggesting an intracellular function.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Recombinant human IL-32α
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-32 recombinant protein ranging from 500 to 7.8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture1: The purified KU32-07 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated KU32-52 antibody (Cat. No. 513503) as the detecting antibody.

Note: For testing human IL-32a in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 433504 & 433505) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Kim KH, et al. 2008. J. Immunol. Methods 333:38. (ELISA Capture)
  2. Kulkarni O, et al. 2008. J. Pharmacol. Exp. Ther. 328:371. PubMed
  3. Greene CM, et al. 2010. AM J. Respir Crit Care Med. 181:31. PubMed
  4. Sakitani K, et al. 2012. Infect. Immun. 80:3795. (ELISA Capture) PubMed
  5. Li Y, et al. 2013. J. Biol. Chem. 288:20927. PubMed
AB_2561289 (BioLegend Cat. No. 513301)
AB_2561290 (BioLegend Cat. No. 513302)

Antigen Details

IL-32 contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence.

Expression increased after activation of T-cells by mitogens or activation of NK cells by IL-2.

IL-32 is a proinflammatory cytokine associated with viral and bacterial infections. IL-32 is involved in autoimmune diseases like rheumatoid arthritis and Crohn disease. IL-32 induces the production of TNF-a from macrophage cells.
Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia.
Proteinase 3 (PR3).
Cell Type
Thymocytes, Tregs
Biology Area
Molecular Family
Antigen References

1. Kim KH, et al. 2008. J. Immunol. Methods 333:38.
2. Conti P, et al. 2007. Autoimmun. Rev. 6:131.
3. Chen Q, et al. 2006. Vitam Horm. 74:207.
4. Kim SH, et al. 2005. Immunity 22:131.
5. Cagnard N, et al. 2005. Eur. Cytokine Netw. 16:289.
6. Banda NK, et al. 2003. J. Immunol. 170:2100.

Gene ID
9235 View all products for this Gene ID
View information about IL-32 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 09.22.2016

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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  • Purified anti-human IL-32α


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