Purified anti-human CD4 Recombinant Antibody

Pricing & Availability
Clone
QA20A34 (See other available formats)
Regulatory Status
RUO
Other Names
T4
Isotype
Rat IgG1, κ
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Product Citations
publications
QA20A34_PURE_CD4_Antibody_111521
Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD4 (clone QA20A34) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 goat anti-rat secondary antibody (clone Poly4054, red) and Alexa Fluor® 647 anti-human CD19 (clone A17136C, green) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was scanned with a 10X objective and stitched with software.
  • QA20A34_PURE_CD4_Antibody_111521
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD4 (clone QA20A34) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 goat anti-rat secondary antibody (clone Poly4054, red) and Alexa Fluor® 647 anti-human CD19 (clone A17136C, green) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was scanned with a 10X objective and stitched with software.
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376402 100 µg 137€
 
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2922599 (BioLegend Cat. No. 376402)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages 

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Monocytes, NKT cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Center D, et al. 1996. Immunol. Today 17:476-81.
  2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723-8.
Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.15.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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  • Purified anti-human CD4 Recombinant Antibody

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