Purified anti-FMRP Antibody

Pricing & Availability
Clone
O91D10 (See other available formats)
Regulatory Status
RUO
Other Names
Fragile X Mental Retardation 1, Fragile X Mental Retardation Protein 1, Synaptic Functional Regulator FMR1, Premature Ovarian Failure 1, Protein FMR-1, FRAXA, POF1, POF
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
O91D10_Purified_FMRP_Antibody_040119
Western blot of purified anti-FMRP antibody (clone O91D10). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • O91D10_Purified_FMRP_Antibody_040119
    Western blot of purified anti-FMRP antibody (clone O91D10). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
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859001 25 µg 72,00€
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859002 100 µg 188,00€
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Description

Fragile X mental retardation protein 1 (FMRP) is an RNA binding protein important for normal cognitive development. In fragile X syndrome, abnormal expansion of CGG trinucleotide repeats in FMR1, the gene that encodes FMRP, leads to methylation and silencing of this gene. Altered FMPR expression levels leads to severe learning deficits or mental retardation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human recombinant protein fragment: amino acids 36-279
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
RRID
AB_2801227 (BioLegend Cat. No. 859001)
AB_2801228 (BioLegend Cat. No. 859002)

Antigen Details

Structure
FMRP is a 632 amino acid protein with a molecular mass of ~71 kD.
Distribution

Tissue Distribution: Ubiquitously expressed with higher expression levels in brain tissue.
Cellular distribution: Plasma membrane, nucleus, and cytosol.

Function
FMRP has been reported to be involved in synaptic plasticity, dendritic spine maturation as well as synapse formation. FMRP may play a role in gene silencing by acting as translation regulator.
Interaction
FMRP interacts with the RNA binding proteins such as FXR1P, FXR2P and 82-FIP. FMRP can also interact with the cytoplasmic proteins CYFIP1 and CYFIP2 which then interacts with eIF4E thus linking FMRP to the RhoGTPase signaling pathway.
Ligand/Receptor
mGluR stimulation leads to increased FMRP expression.
Cell Type
Neurons
Biology Area
Neurodegeneration, Protein Synthesis
Molecular Family
Postsynaptic proteins, Presynaptic proteins
Antigen References
  1. Qiu S, et al. 2018. Mol Brain. 11(1):54
  2. Sunamura N, et al. 2018. Sci Rep. 8(1):11585
  3. Linder B, et al. 2008. Hum Mol Genet. 17(20):3236-46
  4. Gholizadeh S, et al. 2014. Neuropsychopharmacology. 39:3100.
Gene ID
2332 View all products for this Gene ID
UniProt
View information about FMRP on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04.01.2019

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