PE/Cyanine7 anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
BM8_PECy7_122607
Thioglycolate-elicited BALB/c mouse peritoneal macrophages stained with BM8 PE/Cyanine7
  • BM8_PECy7_122607
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages stained with BM8 PE/Cyanine7
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
123113 25 µg 76,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
123114 100 µg 220,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, and Western blotting.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
Product Citations
  1. Oertel S, et al. 2017. Cell Mol Life Sci. 10.1007/s00018-017-2518-9. PubMed
  2. Li S, et al. 2017. Applied Materials & Interfaces. 10.1021/acsami.7b03160. PubMed
  3. Shimoda A, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.07.126. PubMed
  4. Moriyasu T, et al. 2018. PLoS Negl Trop Dis. 12:e0006197. PubMed
  5. Efremova M, et al. 2018. Nat Commun. 9:32. PubMed
  6. Chu LH, et al. 2018. Nat Commun. 9:996. PubMed
  7. Clemente C, et al. 2018. Nat Commun. 9:910. PubMed
  8. Linde N, et al. 2018. Nat Commun. 9:21. PubMed
  9. Youssif C, et al. 2018. EMBO Mol Med. 10:e8403. PubMed
  10. Chung L et al. 2018. Cell host & microbe. 23(2):203-214 . PubMed
  11. Xu X, et al. 2018. Cell. 175:1336. PubMed
  12. Medler TR et al. 2018. Cancer cell. 34(4):561-578 . PubMed
  13. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  14. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  15. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
  16. Laban H, et al. 2018. J Cell Biol. 217:1503. PubMed
  17. Frank AC, et al. 2019. Nat Commun. 10:1135. PubMed
  18. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
  19. Petriello MC, et al. 2018. Toxicol Sci. 162:548. PubMed
  20. Fachi JL et al. 2019. Cell reports. 27(3):750-761 . PubMed
  21. Han Y et al. 2019. Cell reports. 27(3):835-846 . PubMed
  22. Shikatani EA et al. 2019. Cell Rep. 27(8):2304-2312 . PubMed
  23. Li C, et al. 2018. Cell. 174:285. PubMed
  24. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  25. Maston LD, et al. 2017. Am J Physiol Lung Cell Mol Physiol. 312:L609. PubMed
  26. Luo W, et al. 2019. Mol Med Rep. 20:1007. PubMed
  27. Koh EH, et al. 2018. Diabetes. 67(12). PubMed
  28. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  29. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  30. Ponzetta A, et al. 2020. Cell. 178(2):346-360.e24.. PubMed
  31. Su W et al. 2019. Cancer Cell. 36(2):139-155 . PubMed
  32. Wang ECE, et al. 2019. Cell Stem Cell. 24:654. PubMed
  33. Xiong X et al. 2019. Mol Cell. 75(3):644-660 . PubMed
  34. Zhang S, et al. 2019. Cell Metab. 29:443. PubMed
  35. Grayczyk JP et al. 2017. Cell host & microbe. 22(5):678-687 . PubMed
  36. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  37. Nabet BY et al. 2017. Cell. 170(2):352-366 . PubMed
  38. Sierro F, et al. 2017. Immunity. 47:374. PubMed
  39. Poh AR, et al. 2017. Cancer Cell. 31:563. PubMed
  40. Nalbandian A, et al. 2017. Inflammation. 40:21:00. PubMed
  41. Muchenditsi A, et al. 2017. Am J Physiol Gastrointest Liver Physiol. 313:G39. PubMed
  42. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  43. Zhang C, et al. 2018. Cell Res. 28:323. PubMed
  44. Qu J, et al. 2018. J Immunol Res. 2018:7519856. PubMed
  45. Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed
  46. Shang W, et al. 2019. J Diabetes Res. 2019:5641271. PubMed
  47. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  48. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  49. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  50. Currier MA, et al. 2017. Oncotarget. 8:17412. PubMed
  51. Terashima Y, et al. 2020. Nat Commun. 11:609. PubMed
  52. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  53. Fransén Pettersson N, et al. 2018. PLoS One. 13:e0203228. PubMed
  54. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  55. Zheng Z, et al. 2020. Cell Death Dis. 0.684722222. PubMed
  56. Yu YR, et al. 2020. STAR Protocols. 11(3):e0150606. PubMed
  57. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  58. Cai B, et al. 2020. Cell Metabolism. 31(2):406-421.e7.. PubMed
  59. Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed
  60. Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
  61. Liu Z, et al. 2020. STAR Protoc. 1:100029. PubMed
  62. Quenum Zangbede FO, et al. 2018. J Neurosci. 38:6737. PubMed
  63. Torroella-Kouri M, et al. 2009. Cancer Res. 69:4800. PubMed
  64. Fujikura D, et al. 2013. PLoS One. 8:e55321. PubMed
  65. Song M, et al. 2013. Am J Pathol. 182:1308. PubMed
  66. Sharif O, et al. 2013. J Immunol. 190:5640. PubMed
  67. Chan M, et al. 2013. J Cell Sci. 126:3948. PubMed
  68. Patnode M, et al. 2013. J Biol Chem. 288:26533. PubMed
  69. Kong P, et al. 2013. Am J Physiol Heart Circ Physiol. 305:1363. PubMed
  70. Saxena A, et al. 2013. J Immunol. 191:4838. PubMed
  71. Weber G, et al. 2014. J Exp Med. 211:1243. PubMed
  72. Denis C, et al. 2014. Nucleic Acids Res. 42:7370. PubMed
  73. Tay S, et al. 2014. J Immunol. 193:2087. PubMed
  74. Chiu Y, et al. 2014. J Immunol. 193:2207. PubMed
  75. Mojumdar K, et al. 2014. EMBO Mol Med. 6:1476. PubMed
  76. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  77. Giordano C, et al. 2014. Hum Mol Genet. . PubMed
  78. Hamilton J, et al. 2015. J Immunol. 194:5022. PubMed
  79. Doni A, et al. 2015. J Exp Med. 212:905. PubMed
  80. Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
  81. Kim P, et al. 2015. Infect Immun . 83: 2992 - 3002. PubMed
  82. Sive J, et al. 2015. Leukemia. . PubMed
  83. Tozuka M, et al. 2016. J Dermatol Sci. 82: 38-45. PubMed
  84. Stutchfield B, et al. 2015. Gastroenterology. 149: 1896-1909.e14. PubMed
  85. Aguado B, et al. 2015. Sci Rep. 5: 17566. PubMed
  86. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  87. Mangum L, et al. 2016. Toxicol Sci. 150: 169 - 177. PubMed
  88. Jondle C, et al. 2016. J Immunol. 196: 3088-3096. PubMed
  89. Chng S, et al. 2016. Sci Rep. 6: 23820. PubMed
  90. INAGAWA H, et al. 2016. In Vivo. 30: 205 - 211. PubMed
  91. Kwon H, Kim S 2016. Cell Death Dis. 7:22285. PubMed
  92. Orberg E, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.53. PubMed
  93. Maisel D, et al. 2016. PLoS One. 11: 0159716. PubMed
  94. Coppo M, et al. 2016. Nat Commun. 7:12254. PubMed
  95. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
  96. Francis M, et al. 2017. Toxicol Sci. 155(1):182-195. PubMed
  97. Allegrezza M, et al. 2016. Cancer Res . 76: 6253 - 6265. PubMed
  98. Harvey RE et al. 2017. Endocrinology. 158(7):2179-2189 . PubMed
  99. Alonso-Herranz L, et al. 2020. Elife. 9:00. PubMed
  100. Wanner-Seleznik GM, et al. 2020. Immunohorizons. 0.644444444. PubMed
  101. Heim CE, et al. 2020. Nature Microbiology. 5(10):1271-1284. PubMed
  102. Ren Y, et al. 2020. Stem Cell Res Ther. 0.743055556. PubMed
  103. Senatus L, et al. 2020. JCI Insight. 5:00. PubMed
  104. Urbanska AM, et al. 2020. Cancer Biother Radiopharm. 35:459. PubMed
  105. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  106. Li J, et al. 2020. FASEB J. 34:3091. PubMed
  107. Tripathi H, et al. 2020. Stem Cell Rev Rep. 0.953472222. PubMed
  108. Cornax I, et al. 2021. Microorganisms. 9:00. PubMed
  109. Sheng J, et al. 2021. eLife. 10:00. PubMed
  110. Szafran BN, et al. 2021. Chemical Research in Toxicology. 34(6):1556-1571. PubMed
  111. Hall JA, et al. 2020. Cell Metabolism. 32(4):665-675.e6. PubMed
  112. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  113. Yun H, et al. 2020. Immunity. 53(5):1050-1062.e5. PubMed
  114. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  115. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  116. Yamakawa D, et al. 2021. Cell Reports. 34(10):108817. PubMed
  117. Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed
  118. Sangaletti S, et al. 2021. iScience. 24(6):102510. PubMed
  119. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  120. Bae S, et al. 2021. Cell Reports. 35(11):109264. PubMed
  121. Ahn D, et al. 2021. Cell Reports. 35(9):109196. PubMed
  122. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  123. Wedekind MF, et al. 2021. iScience. 24(7):102759. PubMed
  124. Brigas HC, et al. 2021. Cell Reports. 36(9):109574. PubMed
  125. Nabekura T, et al. 2020. Immunity. 96:52. PubMed
  126. Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
  127. Zhang L, et al. 2020. Cell. 442:181. PubMed
  128. Park HB, et al. 2020. Nat Microbiol. 1319:5. PubMed
  129. Damal Villivalam S, et al. 2020. Nat Commun. 4313:11. PubMed
  130. Xu Z, et al. 2020. Cancer Immunol Res. 1354:8. PubMed
  131. Aggen DH, et al. 2020. Clin Cancer Res. . PubMed
  132. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  133. Chavkin NW, et al. 2021. J Am Heart Assoc. 10:e019904. PubMed
  134. Frank AC, et al. 2021. Theranostics. 11:7570. PubMed
  135. Cheng G, et al. 2021. iScience. 24:102673. PubMed
RRID
AB_893490 (BioLegend Cat. No. 123113)
AB_893478 (BioLegend Cat. No. 123114)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 10.25.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account