PE/Cyanine7 anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
17A2_PECy7_083105
C57BL/6 splenocytes stained with 17A2 PE/Cyanine7
  • 17A2_PECy7_083105
    C57BL/6 splenocytes stained with 17A2 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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100219 25 µg 76,00€
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100220 100 µg 180,00€
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Vacca M, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01462. PubMed
  2. Song P, et al. 2018. Int J Mol Med. 41:935. PubMed
  3. Li Z, et al. 2018. Front Immunol. 9:347. PubMed
  4. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  5. Green CD et al. 2018. Developmental cell. 46(5):651-667 . PubMed
  6. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  7. Djokić V, et al. 2019. Front Immunol. 9:2891. PubMed
  8. Chen Z, et al. 2019. Mol Med Rep. 19:3210. PubMed
  9. Marcandalli J et al. 2019. Cell. 176(6):1420-1431 . PubMed
  10. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  11. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  12. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  13. Page N, et al. 2018. Immunity. 48:937. PubMed
  14. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  15. Schneider C, et al. 2018. Cell. 174:271. PubMed
  16. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  17. Luan HH et al. 2019. Cell. 178(5):1231-1244 . PubMed
  18. Kishore M et al. 2017. Immunity. 47(5):875-889 . PubMed
  19. Campana L, et al. 2018. J Immunol. 200:1169. PubMed
  20. Namkoong H, et al. 2018. PLoS Pathog. 14:e1006955. PubMed
  21. Chan YH, et al. 2019. EMBO Mol Med. 11:e10092. PubMed
  22. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  23. Zhao Y, et al. 2020. Front Immunol. 2.572222222. PubMed
  24. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  25. Spevak CC, et al. 2020. Cell Stem Cell. 26(5):755-765. PubMed
  26. Chitu V, et al. 2020. Cell Reports. 30(9):3004-3019. PubMed
  27. Taube C, et al. 2011. PLoS One. 6:e21799. PubMed
  28. Carson W, et al. 2011. PLoS One. 6:e20385. PubMed
  29. Patnode M, et al. 2013. J Biol Chem. 288:26533. PubMed
  30. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  31. Mooney J, et al. 2015. Sci Rep. 5: 14603. PubMed
  32. Chew W, et al. 2016. Nat Methods. 13: 868-874. PubMed
  33. M H, et al. 2016. Sci Rep. 6: 20588. PubMed
  34. Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed
  35. Wessel AW, et al. 2020. Nat Commun. 4.123611111. PubMed
  36. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  37. Blaszczak AM, et al. 2020. Immunometabolism. 2:00. PubMed
  38. Eastman AE, et al. 2020. Cell Rep. 31:107804. PubMed
  39. Reed-Geaghan EG, et al. 2020. J Exp Med. 217:00:00. PubMed
  40. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  41. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  42. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  43. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  44. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  45. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  46. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  47. Zeng W, et al. 2021. STAR Protocols. 2(1):100361. PubMed
RRID
AB_1732068 (BioLegend Cat. No. 100219)
AB_1732057 (BioLegend Cat. No. 100220)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC, IHC-F, IP, ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC, IHC-F, 3D IHC
Alexa Fluor® 488 anti-mouse CD3 17A2 FC, IHC-F, 3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC, ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC, IHC-F, IP, ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F, FC, 3D IHC, SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC, IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-mouse CD3

  • PE anti-mouse CD3

  • Purified anti-mouse CD3

  • Alexa Fluor® 647 anti-mouse CD3

  • Alexa Fluor® 488 anti-mouse CD3

  • Pacific Blue™ anti-mouse CD3

  • Alexa Fluor® 700 anti-mouse CD3

  • PerCP/Cyanine5.5 anti-mouse CD3

  • PE/Cyanine7 anti-mouse CD3

  • APC/Cyanine7 anti-mouse CD3

  • Brilliant Violet 421™ anti-mouse CD3

  • Brilliant Violet 570™ anti-mouse CD3

  • Brilliant Violet 650™ anti-mouse CD3

  • Brilliant Violet 785™ anti-mouse CD3

  • Brilliant Violet 510™ anti-mouse CD3

  • APC anti-mouse CD3

  • Ultra-LEAF™ Purified anti-mouse CD3

  • Brilliant Violet 605™ anti-mouse CD3

  • Alexa Fluor® 594 anti-mouse CD3

  • Brilliant Violet 711™ anti-mouse CD3

  • Biotin anti-mouse CD3

  • PE/Dazzle™ 594 anti-mouse CD3

  • APC/Fire™ 750 anti-mouse CD3

  • Brilliant Violet 750™ anti-mouse CD3

  • TotalSeq™-A0182 anti-mouse CD3

  • TotalSeq™-B0182 anti-mouse CD3

  • Spark Blue™ 550 anti-mouse CD3

  • Spark NIR™ 685 anti-mouse CD3

  • TotalSeq™-C0182 anti-mouse CD3

  • APC/Fire™ 810 anti-mouse CD3

  • PE/Fire™ 640 anti-mouse CD3

  • Spark YG™ 570 anti-mouse CD3

  • PE/Fire™ 700 anti-mouse CD3

  • PE/Cyanine5 anti-mouse CD3

  • Spark Blue™ 574 anti-mouse CD3 Antibody

  • Spark Violet™ 423 anti-mouse CD3

  • PE/Fire™ 810 anti-mouse CD3

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