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PE anti-human EphA2 Antibody

Pricing & Availability
Clone
SHM16 (See other available formats)
Regulatory Status
RUO
Other Names
Ephrin type-A receptor 2 (EPHA2), Epithelial cell kinase, Tyrosine-protein kinase receptor ECK, Myk2, Sek2, EPH receptor A2, eEpithelial cell receptor protein tyrosine kinase, Protein tyrosine kinase, Soluble EPHA2 variant 1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
SHM16_PE_EphA2_Antibody_FC_011713
Human melanoma cell line, A375, was stained with EphA2 (clone SHM16) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
  • SHM16_PE_EphA2_Antibody_FC_011713
    Human melanoma cell line, A375, was stained with EphA2 (clone SHM16) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
See PE spectral data
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356803 25 tests 117€
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356804 100 tests 268€
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Description

Ephrin receptor A2 (EphA2), also known as Eck, is a 130 kD type I transmembrane glycoprotein which belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EphA2 is expressed on epithelial cells, keratinocytes, dendritic/Langerhans cells and melanocytes. It has also been observed in breast, cervical, ovarian, prostate, colon, skin and lung cancers. EphA2 is an oncoprotein which is an essential survival factor for many melanomas. EphA2 plays an important role in angiogenesis and tumor neovascularization through interaction with ephrin-A1, -A3, -A4 and -A5.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Fielding CA et al. 2017. eLife. 6 pii: e22206. PubMed
  2. Lin JG, et al. 2018. Lab Chip. 18:371. PubMed
  3. Torres–Adorno AM, et al. 2019. Oncogene. 38:2135. PubMed
  4. Li Q, et al. 2020. Cell. 182(1):85-97.e16. PubMed
RRID
AB_2561806 (BioLegend Cat. No. 356803)
AB_2561807 (BioLegend Cat. No. 356804)

Antigen Details

Structure
Type I transmembrane glycoprotein, 130 kD, protein- tyrosine kinase family
Distribution
Epithelium (keratinocytes, renal collecting duct cells, carcinomas), dendritic/Langerhans cells, melanocytes
Function
Angiogenesis and tumor neovascularization
Ligand/Receptor
Ephrin-A1, -A3, -A4, -A5
Cell Type
Epithelial cells, Langerhans cells, Dendritic cells
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, Protein Kinases/Phosphatase
Antigen References

1. Pandey A, et al. 1994. J. Biol. Chem. 269:30154.
2. Sulman E, et al. 1997. Genomics 40:371.
3. Pratt R, et al. 2002. Oncogene 21:7690.
4. Udayakumar D, et al. 2011. Oncogene 30:4921.
5. Brantley-Sieders D, et al. 2008. J. Clin. Invest. 118:64.

Gene ID
1969 View all products for this Gene ID
UniProt
View information about EphA2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 02.13.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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