PE anti-human CD334 (FGFR4) Antibody

Pricing & Availability
Clone
4FR6D3 (See other available formats)
Regulatory Status
RUO
Other Names
FGFR4, fibroblast growth factor receptor 4, TKF, FGF R4
Isotype
Mouse IgG1, κ
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Product Citations
publications
4FR6D3_PE_072307
Human FGFR4 transfected NIH/3T3 cells stained with 4FR6D3 PE
  • 4FR6D3_PE_072307
    Human FGFR4 transfected NIH/3T3 cells stained with 4FR6D3 PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
324305 25 tests 88,00€
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324306 100 tests 192,00€
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Description

The 4FR6D3 antibody recognizes human CD334, also known as fibroblast growth factor 4, FGFR4 and TKF. CD334 is a member of the fibroblast growth factor receptor family and a cell surface tyrosine kinase containing three immunoglobulin-like domains. FGFR4 has a predicted molecular weight of approximately 88 kD and is widely expressed in many tissues including the intestine, muscle, heart, cornea, retina, and pancreas, with highest expression in lung and kidney.CD334 binds acidic fibroblast growth factors (FGF8, GFG1, FGF19, FGF6, and FGF2); ligand binding induces mitogenesis and differentiation. CD334 has been shown to interact with STAT1 and STAT3 and is over-expressed in gynecological tumors suggesting a possible role for this receptor in breast and ovarian tumorigenesis. Unique alleles (Arg388) in FGFR4 have been shown to be associated with cancer progression and increased tumor cell motility. The 4FR6D3 antibody has been shown to be useful for flow cytometry.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NIH-3T3 cells transfected with full-length human FGFR4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Heinzle C, et al. 2012. Cancer Res. 72:5767. PubMed.
Product Citations
  1. Vrijens P, et al. 2019. J Gen Virol. 100:583. PubMed
  2. Heinzle C, et al. 2012. Cancer Res. 72:5767. PubMed
RRID
AB_2103670 (BioLegend Cat. No. 324305)
AB_2231698 (BioLegend Cat. No. 324306)

Antigen Details

Structure
Member of the fibroblast growth factor receptor family. Cell surface tyrosine kinase containing three immunoglobulin-like domains. Predicted molecular weight approximately 88 kD.
Distribution

Widely expressed in many tissues including the intestine, muscle, heart, cornea, retina, and pancreas; highest expression in lung and kidney

Function
Ligand binding induces mitogenesis and differentiation; over-expressed in gynecological tumors suggesting a possible role for this receptor in breast and ovarian tumorigenesis. Unique alleles (Arg388) in FGFR4 have been associated with cancer progression
Interaction
STAT1, STAT3
Ligand/Receptor
Binds acidic fibroblast growth factors (FGF8, GFG1, FGF19, FGF6, and FGF2)
Cell Type
Neural Stem Cells
Biology Area
Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Holtrich U, et al. 1991. Proc. Natl. Acad. Sci. USA 88:10411.
2. Partanen J, et al. 1991. EMBO J. 10:1347.
3. Bange J, et al. 2002. Cancer Res. 62:840.
4. Vainikka S, et al. 1992. EMBO J. 11:4273.

Gene ID
2264 View all products for this Gene ID
UniProt
View information about CD334 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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