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PE anti-human CD107a (LAMP-1) Antibody

Pricing & Availability
Clone
H4A3 (See other available formats)
Regulatory Status
RUO
Workshop
P PR-63; BP 473; P P008
Other Names
Lysosome-Associated Membrane Protein 1, LGP-120, LAMP-1
Isotype
Mouse IgG1, κ
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Product Citations
publications
H4A3_PE_090808.jpg
Thrombin-activated human peripheral blood platelets were stained with CD107a (clone H4A3) PE (pink histogram) or mouse IgG1, κ PE (blue histogram).
  • H4A3_PE_090808.jpg
    Thrombin-activated human peripheral blood platelets were stained with CD107a (clone H4A3) PE (pink histogram) or mouse IgG1, κ PE (blue histogram).
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328607 25 tests 87€
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328608 100 tests 202€
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Description

CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40 kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation, CD107a is transferred to the cell membrane surface of activated platelets, activated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107a has been suggested to play a role in the protection of lysosomal membrane from lysosomal hydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surface expression of LAMP-1 has been shown to correlate with CD8+ T cell and NK cell cytotoxicity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human adult adherent peripheral blood cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting8, immunohistochemical staining2, immunofluorescence5,7, and immunoprecipitation5.

This antibody is specific to human LAMP-1. Positive control: Hela cells; LAMP-1 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.

Application References

(PubMed link indicates BioLegend citation)
  1. Misse D, et al. 1999. Blood 93:2454.
  2. Furuta K, et al. 2001. Am. J. Pathol. 159:449. (IHC)
  3. Watanabe A, et al. 2011. J. Biol. Chem. 286:10702. PubMed
  4. Baron Gaillard CL, et al. 2011. Mol. Cell. Biol. 22:5459. PubMed
  5. Hauck CR and Meyer TF. 1997. FEBS Lett. 405:86. (IF, IP)
  6. De Keersmaecker B, et al. 2012. J. Virol. 86:9351. PubMed
  7. Knodler LA, et al. 2010. P. Natl. Acad. Sci. USA. 107:17733. (IF)
  8. Oh J, et al. 2000. Hum. Mol. Genet. 9:375. (WB)
  9. Salio M, et al. 2013 PNAS. 110:4753. PubMed
Product Citations
  1. Willcox CR, et al. 2020. Immunity. 51(5):813-825.e4.. PubMed
  2. Liu R, et al. 2022. Aging Dis. 13:1576. PubMed
  3. Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed
  4. Lamichhane R, et al. 2020. Eur J Immunol. 50:178. PubMed
  5. Chen D, et al. 2021. Dev Cell. 56:3250. PubMed
  6. Kim JT, et al. 2022. Nat Commun. 13:121. PubMed
  7. Gao Y, et al. 2021. Oncogenesis. 10:62. PubMed
  8. Izumi K, et al. 2021. Immunohorizons. 5:687. PubMed
  9. Stevenson EM, et al. 2022. Nat Commun. 13:4888. PubMed
  10. Karampatzakis A, et al. 2021. Front Immunol. 12:641521. PubMed
  11. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  12. Sibener LV et al. 2018. Cell. 174(3):672-687 . PubMed
  13. Chandran P, et al. 2014. J Leukoc Biol. 96:633. PubMed
  14. Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed
  15. Palazzo A, et al. 2018. J Immunol. 200:2304. PubMed
  16. Yao X, et al. 2020. Adv Sci (Weinh). 7:1902938. PubMed
  17. Su X, et al. 2022. J Transl Med. 20:378. PubMed
  18. Schanin J, et al. 2022. Commun Biol. 5:1226. PubMed
  19. Castro I, et al. 2016. J Virol. 90: 5280 - 5291. PubMed
  20. Pugsley H, et al. 2017. Methods. 112:147-156. PubMed
  21. Oyer JL, et al. 2022. Front Immunol. 13:861681. PubMed
  22. Hirama T, et al. 2021. JCI Insight. 6:. PubMed
  23. Lettau M, et al. 2018. Int Immunol. 30:215. PubMed
  24. Pollack RA, et al. 2017. Cell Host Microbe. 1.218055556. PubMed
  25. Li Y, et al. 2022. J Immunol. 208:347. PubMed
  26. Heard A, et al. 2022. Nat Commun. 13:3367. PubMed
  27. Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed
  28. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  29. Katsuyama E, et al. 2020. Cell Reports. 30(1):112-123.e4.. PubMed
  30. Gambacorta V, et al. 2022. Cancer Discov. . PubMed
  31. Pomplun NL, et al. 2021. Cytometry A. 99:278. PubMed
  32. Christensen SR, et al. 2019. J Virol. 93:8. PubMed
  33. Liu CF, et al. 2017. Front Immunol. 1.445833333. PubMed
  34. Plum T, et al. 2020. Immunity. 52(2):404-416. PubMed
  35. Mo YQ, et al. 2022. J Clin Invest. . PubMed
  36. Shen C, et al. 2021. BMC Med. 19:283. PubMed
  37. Li M, et al. 2020. Virol Sin. 35:588. PubMed
  38. Sadallah S, et al. 2016. J Immunol. 197: 1663 - 1671. PubMed
  39. Huang W, et al. 2012. Cell Res. 22:473. PubMed
  40. Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed
  41. Chen PM, et al. 2022. Sci Adv. 8:eabo4271. PubMed
RRID
AB_1186062 (BioLegend Cat. No. 328607)
AB_1186040 (BioLegend Cat. No. 328608)

Antigen Details

Structure
LAMP-1 is a 417 amino acid protein with a molecular mass of 45 kD.
Distribution

Macrophages, epithelial cells, endothelial cells, some tumor cells; located on the luminal side of lysosomes or on the surface of cell membranes

Function
Protect lysosomal membrane from lysosomal hydrolases, adhesion
Ligand/Receptor
Galaptin
Cell Type
Endothelial cells, Epithelial cells, Macrophages
Biology Area
Cell Biology, Immunology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sarafian V, et al. 2006. Arch. Dermatol. Res. 298:7381.
2. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press.
3. Sawada R, et al. 1993. J. Biol. Chem. 268:12675.
4. Chen JW, et al. 1988. J. Biol. Chem. 263:8754.
5. Chen JW, et al. 1986. Biochem. Soc. Symp. 51:97112.

Gene ID
3916 View all products for this Gene ID
UniProt
View information about CD107a on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 10.22.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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