PE anti-Acetylated Lysine Antibody anti-Acetylated Lysine

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Clone: 15G10 (See other available formats)
Regulatory Status: RUO
Other Names: Proteins are acetylated by the reversible transfer of acetyl-CoA to the ε-amino group of lysine
Isotype: Mouse IgG2b, κ
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Product Citations: publications

15G10_PE_acetylated_lysine_Antibody_FC_1_013114 — Human cervical carcinoma cell line, HeLa, was incubated with (top) or without (bottom) Trichostatin A (100 ng/ml, overnight), then treated with FOXP3 Fix/Perm Buffer Set. Cells were then stained with anti-acetylated lysine (clone 15G10) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).

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623404

100 tests

268€

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Description

Proteins are reversibly and dynamically acetylated on the ε-amino group of lysine by acetyltransferases and deacetylated by deacetylases. This post-translational modification can regulate protein function (interactions with other proteins and DNA binding). Histones and transcription factors (PCAF, p53, p300, etc) appear to be the major targets of acetyltransferases. Acetylation is usually associated with chromatin remodeling and transcriptional activation, although in some cases (telomeres) it is associated with gene silencing. The 15G10 antibody recognizes acetylated lysine residues on proteins.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Species independent

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Acetylated protein mixture

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application References (PubMed link indicates BioLegend citation)

Kuo HP, et al. 2010. Sci Signal. 3:Ra9. PubMed

Product Citations

Fujii K, et al. 2021. Int J Mol Sci. 22:. PubMed
Luque–Martin R, et al. 2019. Front Pharmacol. 1.279166667. PubMed

RRID

AB_2563126 (BioLegend Cat. No. 623404)

Antigen Details

Structure: Proteins are acetylated by the reversible transfer of acetyl-CoA to the ε-amino group of lysine
Function: Post-translational modification of proteins by acetyltransferases and deacetylated by deacetylases to regulate protein-protein and protein-DNA interactions
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References: 1. Wang S, et al. 2004. Novartis Found. Symp. 259:238.
2. Legube G, et al. 2003. EMBO Rep. 4:944.
3. Chen LF, et al. 2003. J. Mol. Med. 81:549.
Gene ID: 8850 View all products for this Gene ID
UniProt: View information about Acetylated Lysine on UniProt.org

Documentation

Reviews

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Related Pages & Pathways

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All Acetylated Lysine Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Acetylated Lysine	15G10	WB,ICC
PE anti-Acetylated Lysine	15G10	ICFC
Alexa Fluor® 647 anti-Acetylated Lysine	15G10	ICFC
PE/Cyanine7 anti-Acetylated Lysine	15G10	ICFC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 09.19.2023

For Research Use Only. Not for diagnostic or therapeutic use.

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