- Other Names
- Fsp, Gro1, KC, KC/GRO-alpha, Mgsa, N51, Scyb1
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|740626||100 tests (270 µL)||80€|
The Mouse Anti-Virus Response Panel Mix and Match Capture Beads offer flexible customization within the panel (13-plex) with the following items listed below:
740065 LEGENDplex™ Mouse IFN-γ Capture Bead A4, 13X
740626 LEGENDplex™ Mouse CXCL1 (KC) Capture Bead A5, 13X
740066 LEGENDplex™ Mouse TNF-α Capture Bead A6, 13X
740627 LEGENDplex™ Mouse CCL2 (MCP-1) Capture Bead A7, 13X
740139 LEGENDplex™ Mouse IL-12 (p70) Capture Bead A8, 13X
740628 LEGENDplex™ Mouse CCL5 (RANTES) Capture Bead A10, 13X
740157 LEGENDplex™ Mouse IL-1β Capture Bead B2, 13X
740630 LEGENDplex™ Mouse CXCL10 (IP-10) Capture Bead B3, 13X
740631 LEGENDplex™ Mouse GM-CSF Capture Bead B4, 13X
740632 LEGENDplex™ Mouse IL-10 Capture Bead B5, 13X
740145 LEGENDplex™ Human Mouse IFN-β Capture Bead B6, 13X
740633 LEGENDplex™ Mouse IFN-α Capture Bead B7, 13X
740634 LEGENDplex™ Mouse IL-6 Capture Bead B9, 13X
Selected items will be used together with:
740624 LEGENDplex™ Mouse Anti-Virus Response Panel Standard
740625 LEGENDplex™ Mouse Anti-Virus Response Panel Detection Antibodies
740623 LEGENDplex™ Buffer Set I (Set Up Beads, Buffers, Plate Sealers, SA-PE, Matrix, and Data Analysis Software Dongle)
740377 or 740379 Filter Plate or V-bottom Plate
The LEGENDplex™ Mouse Anti-Virus Response Panel is a bead-based multiplex assay, utilizing fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 13 mouse cytokines, including interferons (α, β, γ), interleukins (1β, 6, 10, 12), chemokines (CCL2 (MCP-1), CCL5 (RANTES), CXCL1 (KC), CXCL10 (IP-10)), TNF-α, and GM-CSF. This assay panel provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. The panel has been validated for use with serum, plasma, and cell culture supernatants.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- If I don't have a vacuum, how do I remove the liquid from my plate?
Do NOT invert the plate to dump the liquid. The beads will not adhere to the plate. After centrifugation using a swing bucket rotor with a plate adaptor, you can remove the liquid by using a pipette.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). FCS files can be analyzed by different software. Data generated from using LEGENDplex™ kits can be analyzed using the LEGENDplex™ data analysis software freely available on the BioLegend website (biolegend.com/legendplex/software). A free of charge software license dongle is currently provided in the kit. Please check our website for the most updated versions of the software.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. Performing the assay with filter plates is generally recommended. A vacuum filtration unit is required to work with the filter plates. If however, you don’t have access to a vacuum manifold, then you can use the V-bottom plates or micro-FACS tubes as described in the manual and follow the recommended assay protocols for the type of plates or tubes you choose. All tubes and plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are valid for 6 months from the date of receipt.