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HRP anti-Apo E Antibody

Pricing & Availability
Clone
D6E10 (See other available formats)
Regulatory Status
RUO
Other Names
Apolipoprotein E, apolipoprotein E3, AD2, LDLCQ5, LPG, ApoE
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
D6E10_HRP_Apo-E_Antibody_1_111218
Western blot of HRP anti-Apo E antibody (clone D6E10). Lane 1: Molecular weight marker; Lane 2: 50 ng of Apo E2 recombinant human protein; Lane 3: 50 ng of Apo E3 recombinant human protein; Lane 4: 50 ng of Apo E4 recombinant human protein; Lane 5: 40 µg of human plasma. The blot was incubated with 1 µg/mL of the primary antibody at 4°C overnight. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • D6E10_HRP_Apo-E_Antibody_1_111218
    Western blot of HRP anti-Apo E antibody (clone D6E10). Lane 1: Molecular weight marker; Lane 2: 50 ng of Apo E2 recombinant human protein; Lane 3: 50 ng of Apo E3 recombinant human protein; Lane 4: 50 ng of Apo E4 recombinant human protein; Lane 5: 40 µg of human plasma. The blot was incubated with 1 µg/mL of the primary antibody at 4°C overnight. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
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803308 100 µg 249€
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Description

Apo-E is a class of apolipoprotein primarily synthesized in the liver, but is also found in other tissues such as brain, kidneys, and spleen. Apo-E is a protein component of serum chylomicron and lipoprotein particles, playing essential roles in cholesterol transportation, homeostasis, and metabolism. The expression of Apo-E is induced by cholesterol-rich diets and hyperlipoproteinemia. In the central nervous system, Apo-E is produced by astrocytes and microglia. The Apo-E-containing lipoprotein particles play important functions in brain injury repair, neurogenesis, and synaptic plasticity. Apo-E has been found to be involved in the regulation of amyloid-β aggregation and clearance. Genetic polymorphisms of Apo-E are associated with neuron degenerative disorders including Alzheimer's disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a peptide sequence corresponding to aa 141-160 of human Apo-E.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB).

Application References

(PubMed link indicates BioLegend citation)
  1. Munoz DG and Feldman H. 2000. CMAJ. 162(1):65-72.
  2. Hagberg JM, et al. 2000. Physiol Genomics. 4(2):101-108.
  3. Saunders AM. 2000. J Neuropathol Exp Neurol. 59(9):751-8.
  4. Golabek AA, et al. 2000. Biophys J. 79(2):1008-15.
  5. Zunarelli E, et al. 2000. Breast Cancer Res Treat. 63(3):193-8.
  6. Zunarelli E, et al. 2000. Clin Neuropathol. 19(1):1-6.
  7. Tomiyama T, et al. 1999. Cell Mol Life Sci. 6(3-4):268-79.
  8. Golabek AA., et al. 1996. J Biol Chem. 271(18):10602-6.
RRID
AB_2783380 (BioLegend Cat. No. 803308)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Gene ID
348 View all products for this Gene ID
UniProt
View information about Apo E on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.14.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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