APC/Cyanine7 anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
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Product Citations
publications
6D5_APCCyanine7_CD19_Antibody_031819_updated.png
C57BL/6 splenocytes were stained with CD3 FITC and CD19 (clone 6D5) APC/Cyaninie7 (left) or Rat IgG2a, ? APC/Cyanine7 isotype control (right).
  • 6D5_APCCyanine7_CD19_Antibody_031819_updated.png
    C57BL/6 splenocytes were stained with CD3 FITC and CD19 (clone 6D5) APC/Cyaninie7 (left) or Rat IgG2a, ? APC/Cyanine7 isotype control (right).
See APC/Cyanine7 spectral data
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115529 25 µg 76,00€
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115530 100 µg 156,00€
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  2. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  3. Xu J et al. 2018. Cell. 173(3):762-775 . PubMed
  4. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
  5. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
  6. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
  7. Proietti M, et al. 2019. Nat Commun. 10:250. PubMed
  8. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  9. Engblom C, et al. 2017. Science. 358:6367. PubMed
  10. Barbet G, et al. 2018. Immunity. 48:584. PubMed
  11. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  12. Perruzza L, et al. 2019. Sci Rep. 9:9315. PubMed
  13. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  14. Tang J, et al. 2018. Mol Cell Biol. 38:e00103. PubMed
  15. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  16. Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed
  17. Stone AEL, et al. 2019. Nat Commun. 10:3649. PubMed
  18. Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed
  19. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
  20. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
  21. Yahagi A, et al. 2019. RMD Open. 5:e000853. PubMed
  22. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  23. Lercher A, et al. 2019. Immunity. 51:1074. PubMed
  24. Rohner L, et al. 2020. Sci Rep. 0.570833333. PubMed
  25. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  26. Liu YG, et al. 2019. FASEB J. 33:5018. PubMed
  27. Quatrini L, et al. 2018. Nat Immunol. 19:954. PubMed
  28. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  29. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  30. Hassan AO, et al. 2020. Cell. 183:169. PubMed
  31. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  32. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  33. Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
  34. Weber G, et al. 2014. J Exp Med. 211:1243. PubMed
  35. Lee M, et al. 2014. PLoS One. 9:112666. PubMed
  36. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  37. Koh F, et al. 2015. Proc Natl Acad Sci U S A. 112:1734. PubMed
  38. Kim P, et al. 2015. J Exp Med. 212:633. PubMed
  39. Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed
  40. Moodley D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9852 - 9857. PubMed
  41. Strawbridge R, et al. 2016. Diabetes. 65: 2888 - 2899. PubMed
  42. Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed
  43. Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed
  44. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
  45. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  46. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  47. Chikina AS, et al. 2020. Cell. 183:411. PubMed
  48. Laidlaw BJ, et al. 2020. Nat Immunol. 1.626388889. PubMed
  49. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  50. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  51. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  52. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  53. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  54. Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed
  55. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  56. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  57. Wei SC, et al. 2020. Cancer Discov. . PubMed
  58. Hu Y, et al. 2021. Cell Death Dis. 12:743. PubMed
RRID
AB_830706 (BioLegend Cat. No. 115529)
AB_830707 (BioLegend Cat. No. 115530)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 03.18.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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