APC/Cyanine7 anti-mouse CD19 Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse CD19-expressing K562 human erythroleukemia cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence⁷.

Additional Product Notes

BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)

1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
12. Olive V, et al. 2013. Elife. 2:822. PubMed
13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed

Product Citations

1. Laidlaw BJ, et al. 2020. Nat Immunol. 1.626388889. PubMed
2. Perruzza L, et al. 2019. Sci Rep. 9:9315. PubMed
3. Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed
4. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
5. Pani F, et al. 2021. Endocrinology. 162:. PubMed
6. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
7. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
8. Proietti M, et al. 2019. Nat Commun. 10:250. PubMed
9. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
10. Ercoli G, et al. 2021. Front Immunol. 11:611661. PubMed
11. Machata S, et al. 2021. Front Immunol. 11:565869. PubMed
12. Moodley D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9852 - 9857. PubMed
13. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
14. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
15. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
16. Tang J, et al. 2018. Mol Cell Biol. 38:e00103. PubMed
17. Quatrini L, et al. 2018. Nat Immunol. 19:954. PubMed
18. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
19. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
20. Chikina AS, et al. 2020. Cell. 183:411. PubMed
21. Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed
22. Tilstam PV, et al. 2021. J Clin Invest. 131:. PubMed
23. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
24. Trott DW, et al. 2021. Geroscience. 43:1331. PubMed
25. Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed
26. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
27. Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed
28. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
29. Bresque M, et al. 2022. J Biol Chem. :101711. PubMed
30. Mukherjee D, et al. 2022. Nat Commun. 13:3747. PubMed
31. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
32. Lercher A, et al. 2019. Immunity. 51:1074. PubMed
33. Stone AEL, et al. 2019. Nat Commun. 10:3649. PubMed
34. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
35. Lee M, et al. 2014. PLoS One. 9:112666. PubMed
36. Koh F, et al. 2015. Proc Natl Acad Sci U S A. 112:1734. PubMed
37. Wei SC, et al. 2020. Cancer Discov. . PubMed
38. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
39. Ghilas S, et al. 2021. iScience. 24:103059. PubMed
40. Kim P, et al. 2015. J Exp Med. 212:633. PubMed
41. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
42. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
43. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
44. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
45. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
46. Weber G, et al. 2014. J Exp Med. 211:1243. PubMed
47. Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed
48. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
49. Xu J et al. 2018. Cell. 173(3):762-775 . PubMed
50. Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
51. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
52. Akazawa S, et al. 2021. Diabetologia. 64:878. PubMed
53. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
54. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
55. Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed
56. Pitsch J, et al. 2021. Ann Neurol. 89:666. PubMed
57. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
58. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
59. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
60. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
61. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
62. Rohner L, et al. 2020. Sci Rep. 0.570833333. PubMed
63. Hassan AO, et al. 2020. Cell. 183:169. PubMed
64. Carbone C, et al. 2021. J Immunother Cancer. 9:. PubMed
65. Elkhatib SK, et al. 2021. Biol Psychiatry Glob Open Sci. 1:190. PubMed
66. Colombo M, et al. 2022. iScience. 25:105042. PubMed
67. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
68. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
69. Yahagi A, et al. 2019. RMD Open. 5:e000853. PubMed
70. Hu Y, et al. 2021. Cell Death Dis. 12:743. PubMed
71. Fischer FA, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
72. Trott DW, et al. 2021. J Physiol. 599:3973. PubMed
73. Engblom C, et al. 2017. Science. 358:6367. PubMed
74. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
75. Barbet G, et al. 2018. Immunity. 48:584. PubMed
76. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
77. Liu YG, et al. 2019. FASEB J. 33:5018. PubMed
78. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
79. Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed
80. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
81. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
82. Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed
83. Du P, et al. 2022. Front Pharmacol. 13:805508. PubMed
84. Strawbridge R, et al. 2016. Diabetes. 65: 2888 - 2899. PubMed
85. Yoshida H, et al. 2019. Cell. 176:897. PubMed
86. La Salvia S, et al. 2020. Am J Physiol Renal Physiol. 319:F868. PubMed
87. Katzmarski N, et al. 2021. Nat Immunol. 22:1382. PubMed
88. Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed
89. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
90. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
91. Guan D, et al. 2021. Cell Death Dis. 12:431. PubMed
92. Chavez JS, et al. 2022. Cells. 11:. PubMed
93. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
94. Wilson CS, et al. 2021. JCI Insight. 6:. PubMed
95. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
96. Habener A, et al. 2021. J Allergy Clin Immunol. 147:2281. PubMed
97. Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed
98. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
99. Cheng Q, et al. 2020. Eur J Immunol. 50:284. PubMed

RRID

AB_830706 (BioLegend Cat. No. 115529)
AB_830707 (BioLegend Cat. No. 115530)

Antigen Details

Structure

Ig superfamily, associates with CD21 and CD81, 95 kD

Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function

Modulates B cell activation and differentiation

Ligand/Receptor

CD21, CD81, Leu-13

Cell Type

B cells, Dendritic cells

Biology Area

Costimulatory Molecules, Immunology

Molecular Family

CD Molecules

Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID

12478 View all products for this Gene ID

UniProt

View information about CD19 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis