APC/Cyanine7 anti-mouse CD117 (c-kit) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse bone marrow mast cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunohistochemistry of acetone fixed frozen sections², and spatial biology (IBEX)^5,6. The 2B8 antibody does not block c-Kit activity.

Additional Product Notes

BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)

1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
4. Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

1. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
2. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
3. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
4. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
5. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
6. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
7. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
8. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
9. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
10. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
11. Iturri L, et al. 2021. Immunity. . PubMed
12. Wu X, et al. 2016. Nat Commun. 7:10533. PubMed
13. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
14. Wu HC, et al. 2021. Cancer Discov. Online ahead of print. PubMed
15. Li Z, et al. 2022. Elife. 11:. PubMed
16. Taylor D, et al. 2011. J Immunol. 186:5956. PubMed
17. Nguyen DTT, et al. 2020. Nat Commun. 11:2026. PubMed
18. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
19. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
20. Khoramian Tusi B, et al. 2018. Exp Hematol. 60:21. PubMed
21. Nurkhametova D, et al. 2019. Front Cell Neurosci. 13:45. PubMed
22. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
23. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
24. Li Y, et al. 2021. Nat Commun. 0.843055556. PubMed
25. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
26. Yu VWC, et al. 2017. Cell. 168:944. PubMed
27. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
28. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
29. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
30. Bell CC, et al. 2019. Nat Commun. 10:2723. PubMed
31. Reeh K, et al. 2014. Development. 141:2950. PubMed
32. Beyaz S, et al. 2016. Nature. 531:53-58. PubMed
33. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
34. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
35. Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed
36. Carr M, et al. 2016. Proc Natl Acad Sci U S A. 113(52):15024-15029. PubMed
37. Comoglio F, et al. 2018. Genome Res. 10.1101/gr.227272.117. PubMed
38. Cheng Y, et al. 2019. Cell Rep. 28:1703. PubMed
39. Ku C, et al. 2015. Genes Dev. 29: 1930-1941. PubMed
40. Minuesa G, et al. 2019. Nat Commun. 10:2691. PubMed
41. Sevin M, et al. 2018. Nat Commun. 9:1431. PubMed
42. Ding P, et al. 2022. Bone Res. 10:42. PubMed
43. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
44. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
45. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
46. Cazares O, et al. 2021. Development. 148:. PubMed
47. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
48. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
49. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
50. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
51. Wang S, et al. 2021. Commun Biol. 22:. PubMed
52. Mana MD, et al. 2021. Cell Reports. 35(10):109212. PubMed
53. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
54. Abdala-Valencia H, et al. 2015. J Immunol. 195: 1377-1387. PubMed
55. Rüder C, et al. 2014. PLoS One. 9:104644. PubMed
56. Stine RR, et al. 2020. Cell Stem Cell. 25(6):830-845. PubMed
57. Bikorimana E, et al. 2014. J Vis Exp. 92: 52022. PubMed
58. Shigeta A, et al. 2019. Dev Cell. 48:617. PubMed
59. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
60. Koide S, et al. 2022. iScience. 25:103603. PubMed
61. Kassas–Guediri A, et al. 2017. PLoS One. 12:e0173815. PubMed
62. Krivtsov AV, et al. 2020. Cancer Cell. 36(6):660-673.e11.. PubMed
63. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
64. Ortega-Molina A, et al. 2021. Cell Reports. 36(2):109372. PubMed
65. Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed
66. Fransén Pettersson N, et al. 2018. PLoS One. 13:e0203228. PubMed
67. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
68. Ito K et al. 2019. Cell Rep. 28(10):2480-2490 . PubMed
69. Li X, et al. 2019. Stem Cells. 37:937. PubMed
70. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
71. Morganti C, et al. 2022. EMBO Rep. 23:e54262. PubMed
72. Tu Z, et al. 2022. Cell Rep. 40:111072. PubMed
73. Chavez JS, et al. 2022. Cells. 11:. PubMed
74. Fielding C, et al. 2022. Nat Commun. 13:543. PubMed
75. Lee SG, et al. 2020. J Nucl Med. . PubMed
76. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
77. Park SM et al. 2018. Cell stem cell. 24(1):153-165 . PubMed
78. Chaudhury S, et al. 2018. Nat Commun. 9:5280. PubMed
79. Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed
80. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
81. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
82. Jee D et al. 2018. Molecular cell. 69(2):265-278 . PubMed
83. Dingler FA, et al. 2020. Mol Cell. . PubMed
84. Young K, et al. 2021. Cell Stem Cell. . PubMed
85. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed

RRID

AB_1626280 (BioLegend Cat. No. 105825)
AB_1626278 (BioLegend Cat. No. 105826)

Antigen Details

Structure

Ig superfamily, 145 kD

Distribution

Hematopoietic stem cells, AML, mast cells

Function

Growth factor receptor, tyrosine kinase

Ligand/Receptor

Stem Cell Factor (SCF)

Cell Type

Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells

Biology Area

Immunology, Stem Cells

Molecular Family

CD Molecules

Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

Gene ID

16590 View all products for this Gene ID

UniProt

View information about CD117 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis