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APC anti-mouse CD45.1 Antibody

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
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Product Citations
publications
A20_APC_090507
SJL mouse splenocytes stained with A20 APC
  • A20_APC_090507
    SJL mouse splenocytes stained with A20 APC
Compare all formats See APC spectral data
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110713 25 µg 36€
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110714 100 µg 102€
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Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
  2. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  3. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  4. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  5. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  6. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
  7. Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed
  8. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  9. Sano T, et al. 2021. Cell Reports. 36:109608. PubMed
  10. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  11. Bangs DJ, et al. 2022. Cell Rep. 38:110266. PubMed
  12. Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed
  13. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  14. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  15. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  16. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  17. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  18. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  19. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  20. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  21. Bhattacherjee A, et al. 2021. Mol Neurodegener. 16:19. PubMed
  22. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  23. Pawaria S, et al. 2020. Arthritis Rheumatol. 72:359. PubMed
  24. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  25. Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
  26. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  27. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  28. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  29. García-Bonilla M, et al. 2020. Stem Cell Res Ther. 11:121. PubMed
  30. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  31. Marchingo JM, et al. 2020. eLife. 9:e53725.. PubMed
  32. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  33. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
  34. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  35. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  36. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  37. Li J, et al. 2020. Elife. 9:00. PubMed
  38. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
  39. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  40. van Montfoort N, et al. 2018. Cell. 175:1744. PubMed
  41. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  42. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  43. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  44. Port JR, et al. 2020. J Virol. 94:. PubMed
  45. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  46. Korrer M, Routes J 2014. PLoS One. 9:91370. PubMed
  47. Wang L, et al. 2014. Proc Natl Acad Sci U S A. 111:3146. PubMed
  48. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  49. Taylor D, et al. 2022. Front Immunol. 13:936129. PubMed
  50. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  51. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  52. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  53. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  54. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
  55. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  56. Sato K, et al. 2017. J Immunol. 10.4049/jimmunol.1602005. PubMed
  57. Wu J, et al. 2021. STAR Protoc. 2:101022. PubMed
  58. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  59. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  60. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  61. Ocaña-Morgner C, et al. 2013. J Immunol. 190:5545. PubMed
  62. Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
  63. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  64. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  65. Endo-Umeda K, et al. 2021. Sci Rep. 11:22595. PubMed
  66. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  67. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
  68. Akane K, et al. 2016. Proc Natl Acad Sci U S A. 113: 2460 - 2465. PubMed
  69. Hu Z, et al. 2013. J Immunol. 191:312. PubMed
  70. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  71. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  72. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  73. Alterauge D, et al. 2020. Cell Rep. 33:108232. PubMed
  74. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  75. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
RRID
AB_313502 (BioLegend Cat. No. 110713)
AB_313503 (BioLegend Cat. No. 110714)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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