Alexa Fluor® 647 anti-Histone H3.1 Phospho (Ser28) Antibody

Pricing & Availability
Clone
5D10D4 (See other available formats)
Regulatory Status
RUO
Other Names
Histone-H3
Isotype
Rat IgG2a, κ
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Product Citations
publications
5D10D4_A647_Histone_H3point1_Phospho_Antibody_1_062416
HeLa cells were stimulated with (top) or without (bottom) nocodozole for 24 hours, fixed and permeabilized with cold 70% ethanol, then intracellularly stained with DAPI and Histone H3.1 Phospho (Ser28) antibody (clone 5D10D4) Alexa Fluor® 647.
  • 5D10D4_A647_Histone_H3point1_Phospho_Antibody_1_062416
    HeLa cells were stimulated with (top) or without (bottom) nocodozole for 24 hours, fixed and permeabilized with cold 70% ethanol, then intracellularly stained with DAPI and Histone H3.1 Phospho (Ser28) antibody (clone 5D10D4) Alexa Fluor® 647.
  • 5D10D4_A647_Histone_H3point1_Phospho_Antibody_2_062416
See Alexa Fluor® 647 spectral data
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687603 25 tests 140,00€
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687604 100 tests 308,00€
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Description

Histone proteins are classified into core histones (H2A, H2B, H3, H4) and linker histones (H1, H5). Core histones form an octamer, which contains two H2A-H2B dimers and one H3-H4 tetramer. Core histones are predominantly globular except for the unstructured N-terminal tails. Posttranslational modifications, such as acetylation, methylation, phosphorylation, ubiquitination, SUMOylation and ADP-ribosylation occur in histone tails.

Histone modifications induce changes of chromatin structure and thereby affect the accessibility of transcription factors, nuclear proteins and enzymes to genomic DNA, resulting in gene activation or repression. It is known that histone modifications play critical roles in DNA repair, DNA replication, transcription regulation, alternative splicing and chromosome condensation and some diseases including autoimmune diseases and cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Histone H3.1 S28ph peptide (21-39)(ATKAARK(phS)APATGGVKKPH), Freund's complete adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
RRID
AB_2616946 (BioLegend Cat. No. 687603)
AB_2616947 (BioLegend Cat. No. 687604)

Antigen Details

Structure
H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
Distribution

Nucleus.

Function
H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Therefore, histones limit the accessibility of DNA by providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.
Interaction
Two molecules of H3 form a heterotetramer with two molecules of H4.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability
Molecular Family
Phospho-Proteins
Antigen References

1. Choi HS, et al. 2005. J. Biol. Chem. 280:13545.
2. Goto H, et al. 2002. Genes Cells 7:11.
3. Garcia BA, et al. 2005. Biochemistry 44:13202.
4. Yoshimi T, et al. 2013. Monoclon. Immunodiagn. Immunother. 32:119.

Gene ID
8350 View all products for this Gene ID
UniProt
View information about Histone H3.1 Phospho Ser28 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 08.16.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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