- 6H2.B4 (See other available formats)
- Regulatory Status
- Other Names
- Cyt c
- Mouse IgG1, κ
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- Product Citations
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Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 6H2.B4 monoclonal antibody recognizes human, mouse, and rat cytochrome-c and has been shown to be useful for intracellular flow cytometric staining, Western blotting, immunoprecipitation, and immunofluorescence staining.Product Details
- Mouse, Rat, Human
- Antibody Type
- Host Species
- Rat cyt c-OVA
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
ICC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: intracellular flow cytometry5, immunofluorescence microscopy3,5, immunoprecipitation4, and immunocytochemistry5.
(PubMed link indicates BioLegend citation)
- Goshorn SC, et al. 1991. J. Biol. Chem. 266:2134.
- Jemmerson R, et al. 1991. Eur. J. Immunol. 21:143.
- Chandra D, et al. 2002. J. Biol. Chem. 277:50842. (IF)
- Semenkova L, et al. 2003. Eur. J. Biochem. 270:4388. (IP)
- Shih S-F, et al. 2001. J. Biol. Chem. 276:21870. (ICFC ICC IF)
- She P, et al. 2011. Am J. Physiol Endcorinol Metab.301:E49. PubMed
- McGuire, KA., et al. 2011. J. Virol 85:10806. PubMed
- Product Citations
AB_2565241 (BioLegend Cat. No. 612310)
- Heme binding domain; 15 kD
Mitochondrial intermembrane space
- Component of electron transport chain; heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9
- Cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, Casp9
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Mitochondrial Markers
- Antigen References
1. Liu X, et al. 1996. Cell. 86:147.
2. Li P, et al. 1997. Cell. 91:479.
3. Zhang Z, et al. 2003. Gene 312:61.
4. Ferguson H, et al. 2003. J. Biol. Chem. 278:45793.
- Gene ID
- 1355 View all products for this Gene ID
- View information about Cytochrome c on UniProt.org
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