In recent years, the involvement of immune cells in controlling cancers has come to the forefront of therapy and research. It is now evident that immune cells are central to the three "E's" of cancer immunoediting: Elimination, Equilibrium, and Escape, as tumors devise a diverse set of mechanisms to overcome immune control. One critical mechanism is the expression of immune checkpoint ligands on tumors that engage checkpoint receptors on immune cells resulting in immune inactivation and tumor escape. Targeting this pathway with blocking antibodies has shown real promise, but continued research is essential. BioLegend provides an extensive array of research tools for immuno-oncology research and continues our commitment as a preferred supplier to the Parker Institute for Cancer Immunotherapy. Discover our enabling tools below.
Accurate immune status monitoring of cancer patients is critical during immunotherapy as well as other oncology therapies. BioLegend provides 12,000 purified and fluorophore conjugated antibodies for flow cytometry with extensive quality testing for flow cytometry. Our selection of fluorophores includes the family of Brilliant Violet™ fluorophores, including BV421™, and our novel fluorophores PE/Dazzle™ 594 and APC/Fire™ 750. Our antibody clones are often provided with a large selection of fluorophore options, allowing for flexibility in developing the best flow cytometry panels. Learn more about our flow cytometry tools.
Con A (three-days) activated C57BL/6 splenocytes were stained with CD3 PE and CD279 (clone RMP1-30) PE/Dazzle™ 594 (left) or rat IgG2b, κ PE/Dazzle™ 594 isotype control (right).
Accurate controls are essential for long-term studies and reproducibility across research sites. Reference laboratories, clinical research organizations, and multi-center clinical trials, among other institutions, need reliable controls to monitor assay performance and variability for longitudinal studies. Our unique lyophilized control cell products: Veri-Cells™ PBMC and CD4-Low PBMC are prepared from human peripheral blood, available as controls to monitor normal and low levels of CD4+ cells. Veri-Cells™ Leukocyte is an excellent human immunophenotyping control, as it includes all leukocyte subsets (lymphocytes, monocytes and granulocytes).
Learn more about Veri-Cells™.
Cell surface staining of various markers on Veri-Cells™ PBMCs.
For researchers interested in no-hassle bulk quantities of biofunctional antibodies for mouse injection or human ex vivo studies, GoInVivo™ provides the best solution at exceptional prices. GoInVivo™ products focus on targeting immune checkpoints. Tumor cells can express high levels of these checkpoint receptors, shutting down immune responses and taking advantage of the lack of inflammation.
Anti-human PD-1 inhibits the binding of PD-L1. Immobilized PD-1-Fc was pre-incubated with increasing concentrations of anti-human PD-1 (clone EH12.2H7, purple squares) or isotype control (clone MOPC-21, purple circles), followed by incubation with a fixed concentration of PD-L1-Fc (1 µg/mL). Clone EH12.2H7 inhibits PD-1/PD-L1 interaction in a dose dependent manner.
Learn more about GoInVivo™.
Soluble MHC molecules are commonly used to identify antigen-specific T cells that react to cancers. Flex-T™ is BioLegend's technology to study antigen-specific T cells through their TCRs. It has the unique property of allowing the loading of peptides of interest into the binding site of the MHC groove, by using ultraviolet (UV) light labile, exchangeable peptides. Flex-T™ technology features:
Learn more about Flex-T™.
Flex-T™ is made of MHC monomers loaded with a peptide that can be degraded by the use of a UV light source. This allows for a peptide exchange when the UV irradiation is done in the presence of the peptide of interest (which is not UV-labile). This flexibility permits the screening of virtually any peptide of interest with enough affinity for the MHC allele that it is loaded onto.
MojoSort™ is BioLegend's magnetic cell separation system for the isolation and purification of cells from heterogeneous populations. MojoSort™ offers outstanding performance at an excellent price.
Separation of cells can occur via "Positive Selection" or "Negative Selection" (also known as "Negative Depletion"). This depends on whether the bead-bound antibodies directly target your cells of interest (positive selection) or target unwanted cells (negative selection). Either way, both separated fractions of cells can be used for downstream applications.
MojoSort™ Nanobeads are magnetic particles directly conjugated to antibodies (positive selection) or Streptavidin. MojoSort™ Isolation Kits typically contain a biotin-antibody cocktail and Streptavidin Nanobeads, intended to isolate an untouched cell population. To use these products, you also need a separation buffer and a magnetic separation system. We recommend our MojoSort™ Buffer (Cat. No. 480017) and MojoSort™ Magnet (Cat. No. 480019/480020).
Learn more about MojoSort™.
A single cell suspension from pooled C57BL/6 mouse spleen and lymph nodes was prepared to isolate CD4+ T cells using the MojoSort™ Mouse CD4 T Cell Isolation Kit. Cells were stained with PE anti-mouse CD4 (clone RM4-4), APC anti-mouse CD3ε (145-2C11), and 7-AAD. Dead cells were excluded from the analysis.
BioLegend provides an extensive selection of bioactive recombinant proteins for cancer research, including cytokines, growth factors, chemokines, and enzymes, such as matrix metalloproteinases. Products are manufactured by BioLegend and quality tested for bioactivity comparable to or better than leading competitors.
Learn more about our Recombinant Proteins.
In cancer states, measuring soluble biomarkers is important in understanding immune states, tumor status, or tumor environments. BioLegend's LEGENDplex™ bead-based immunoassays quantify multiple soluble analytes simultaneously in biological samples using a flow cytometer. LEGENDplex™ kits are provided with predefined panels, ranging from 3 to 13 specificities, or customers can mix and match any subset within each predefined panel using our Mix and Match system.
Learn more about LEGENDplex™.
Phosphorylation, a mechanism by which phosphoryl groups are added to proteins, is important in a number of cancer-related processes. It is involved in the control of proliferation, oncogenic kinase signaling, transcriptional regulation, p53 tumor suppressing activity, and many other processes. BioLegend offers a wide array of phospho protein-specific antibody products for use in flow cytometry, western blot, and microscopy applications. Whenever possible, the phospho protein-specific antibodies we offer are optimized to work for multiple applications, allowing for clone and product consistency across different experimental systems.
Learn more and view our complete selection of phospho-specific antibodies.
Alexa Fluor® 647 anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody (clone 4B11B69)
Intracellular Flow Cytometry (ICFC):
Human peripheral blood lymphocytes were stimulated with (filled histogram), or without (open histogram), Cell Activation Cocktail (without Brefeldin A) for 15 minutes, then fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with ERK1/2 Phospho (Thr202/Tyr204) (Clone 4B11B69) Alexa Fluor® 647
C57BL/6 mouse splenocytes were stimulated with (filled histogram) or without (open histogram) Cell Activation Cocktail (without Brefeldin A) for 15 minutes, then fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with ERK1/2 Phospho (Thr202/Tyr204) (Clone 4B11B69) Alexa Fluor® 647.
Microscopy has become a valuable tool for assessing various tissues and cell types for markers of interest, particularly for monitoring phenotypes of cancerous cells. We provide an extensive selection of directly conjugated antibodies as well as non-antibody chemical probes that assess a variety of functions like viability and proliferation. View our reagents…
HeLa cells were fixed, permeabilized, blocked, and then intracellularly stained with purified PARP (clone 5A5) (red) and followed by anti-mouse IgG-DyLight™ 594 and Alexa Fluor® 488 Phalloidin (green) staining. Nuclei were counterstained with DAPI (blue). The image was captured with a 40x objective.
This webinar, presented by Dr. Robert Schreiber, addresses how mouse and human cancers have been used to identify therapeutically useful tumor neoantigens; how these neoantigens can form the basis for personalized cancer vaccines; and what additional forms of therapy are needed to optimize the therapeutic effects of personalized cancer vaccines.
This seminar, presented by Miguel Tam, Senior Product Manager, discusses addressing cancer research using multiple techniques and tools. Specifically, he addresses in vitro models for characterizing melanoma RANK-RANKL interactions, such as cell migration, intracellular signaling, and immunofluorescence microscopy. PD-L1 blocking antibodies are also investigated in an in vivo mouse model, subsequently examined by T cell activation, cytokine and chemokine production, tumor specific T cells, and tumor growth.